Key features and details
- Rabbit polyclonal to Caspase-9 p35
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Caspase-9 p35 antibody
DescriptionRabbit polyclonal to Caspase-9 p35
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Dog
- RSC96 cells; human colon carcinoma and rat brain tissues; Raji lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab210611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
|Flow Cyt||1/20 - 1/100.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
- Information by UniProt
- APAF-3 antibody
- Apoptotic protease Mch-6 antibody
- Apoptotic protease-activating factor 3 antibody
Anti-Caspase-9 p35 antibody (ab210611) at 1/300 dilution + Raji lysate
Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 46 kDa
Flow cytometric analysis of RSC96 cells labeling Caspase-9 p35 with ab210611 at 1/100 dilution for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Caspase-9 p35 with ab210611 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
ab210611 has not yet been referenced specifically in any publications.