Key features and details
- Rabbit polyclonal to Caspase-9 (phospho T125)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caspase-9 (phospho T125) antibody
See all Caspase-9 primary antibodies
DescriptionRabbit polyclonal to Caspase-9 (phospho T125)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Caspase-9 (phospho T125) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R).
Database link: P55211
- HeLa whole cell lysate (ab150035) and Human lung carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
- Information by UniProt
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
All lanes : Anti-CDC25 (phospho S916) antibody (ab192816) at 1/500 dilution
Lane 1 : HeLa cell lysate with antigen-specific peptide
Lane 2 : HeLa cell lysate
Predicted band size: 46 kDa
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Caspase-9 (phospho T125) using ab192815 at a 1/50 dilution, the right panel was treated with blocking peptide.
ab192815 has not yet been referenced specifically in any publications.