Key features and details
- Rabbit polyclonal to Caspase-9 (phospho Y153)
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caspase-9 (phospho Y153) antibody
See all Caspase-9 primary antibodies
DescriptionRabbit polyclonal to Caspase-9 (phospho Y153)
SpecificityDetects Caspase-9 only when phosphorylated at tyrosine 153.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Caspase-9.
Database link: P55211
- ICC: HeLa cells; WB: extracts from COLO205 cells; IHC-P: thymus tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similaritiesBelongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
- Information by UniProt
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
All lanes : Anti-Caspase-9 (phospho Y153) antibody (ab79202) at 1/500 dilution
Lane 1 : COLO205 cell extracts
Lane 2 : COLO205 cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 40 kDa (possible cleavage fragment)
Left panel: immunofluorescence analysis of HeLa cells using 1/500 ab89202. Right panel: same, after pre-incubation with the immunizing phosphopeptide.
Left panel: immunohistochemistry analysis of paraffin-embedded Human thymus tissue using 1/50 ab79202. Right panel: same, after pre-incubation with the immunizing phosphopeptide.
ab79202 has been referenced in 1 publication.
- Gong W et al. Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a. J Cell Biochem 120:836-847 (2019). PubMed: 30125989