Overview

  • Product name

    Caspase Family Colorimetric Substrate Kit II Plus
    See all Caspase Family kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Product overview

    Abcam's Caspase Family Colorimetric Substrate Kit II Plus provides ready-to-use colorimetric substrates for members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
    Visit our FAQs page for tips and troubleshooting.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 25 tests
    2X Reaction Buffer 1 x 20ml
    Caspase 6 Substrate, Ac-VEID-PNA 1 x 125µl
    Caspase-1 Substrate, Ac-YVAD-pNA 1 x 125µl
    Caspase-10 Substrate, Ac-AEVD-PNA 1 x 125µl
    Caspase-2 Substrate, Ac-VDVAD-pNA 1 x 125µl
    Caspase-3 Substrate, Ac-DEVD-pNA 1 x 125µl
    Caspase-4 Substrate, Ac-LEVD-PNA 1 x 125µl
    Caspase-5 Substrate, Ac-WEHD-pNA 1 x 125µl
    Caspase-8 Substrate, Ac-IETD-pNA 1 x 125µl
    Caspase-9 Substrate, Ac-LEHD-pNA 1 x 125µl
    Cell Lysis Buffer 1 x 100ml
    Dilution Buffer 1 x 200ml
    DTT 1 x 400µl
  • Research areas

  • Relevance

    Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • CASP
    • CASP 2
    • CASP1
    • CASP2
    • Caspase
    • Caspase 1
    • Caspase 1 apoptosis related cysteine peptidase
    • Caspase 2
    • Caspase 2 apoptosis related cysteine peptidase
    • ICE
    • ICH 1 protease
    • ICH 1L
    • ICH 1L/1S
    • ICH1
    • IL 1 beta converting enzyme
    • IL1BC
    • Interleukin 1 B converting enzyme
    • Interleukin 1 beta convertase
    • Interleukin 1 beta converting enzyme
    • NEDD 2
    • NEDD2
    • NEDD2 apoptosis regulatory
    • Neural precursor cell expressed developmentally down regulated 2
    • Neural precursor cell expressed developmentally down regulated protein 2
    • P45
    • Protease ICH 1
    see all

Protocols

References

ab102488 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you very much for contacting us with your questions.

We do have some panels for caspase activity measurement:

1) Ab102486: Includes substrates for caspases 1,2,3,5,6,8, and 9. It does not include substrates for caspases 4 and 7. There are enough reagents included to run 25 assays.

2) Ab102487: Includes substrates for caspases 1,2,3/7,4,5,6,8,9, and 10. Please note that caspases 3 and 7 will be measured together. There are enough reagents included to run 25 assays.

3) Ab102488: Includes substrates for caspases 1,2,3,4,5,6,8, and 9. It does not include substrate for caspases 7. There are enough reagents included to run 25 assays.

Would one of these kits work for you? It doesn't seem like they are exactly what you had in mind but they are the closest products that we currently carry in our catalog.

I hope that this information will be useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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