Caspase Family Fluorometric Substrate Kit II Plus (ab102487)

Overview

  • Product name
    Caspase Family Fluorometric Substrate Kit II Plus
    See all Caspase Family kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Enzyme activity
  • Product overview

    Abcam's Caspase Family Fluorometric Substrate Kit II Plus provides ready-to-use fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
    Visit our FAQs page for tips and troubleshooting.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 25 tests
    2X Reaction Buffer 1 x 20ml
    Caspase-1 Substrate, Ac-YVAD-AFC 1 x 125µl
    Caspase-10 Substarte, Ac-AEVD-AFC 1 x 125µl
    Caspase-2 Substrate, Ac-VDVAD-AFC 1 x 125µl
    Caspase-3/7 Substrate, Ac-DEVD-AFC 1 x 125µl
    Caspase-4 Substrate, Ac-LEVD-AFC 1 x 125µl
    Caspase-5 Substrate, Ac-WEHD-AFC 1 x 125µl
    Caspase-6 Substrate, Ac-VEID-AFC 1 x 125µl
    Caspase-8 Substrate, Ac-IETD-AFC 1 x 125µl
    Caspase-9 Substrate, Ac-LEHD-AFC 1 x 125µl
    Cell Lysis Buffer 1 x 100ml
    DTT 1 x 0.4ml
  • Research areas
  • Relevance
    Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • CASP
    • CASP 2
    • CASP1
    • CASP2
    • Caspase
    • Caspase 1
    • Caspase 1 apoptosis related cysteine peptidase
    • Caspase 2
    • Caspase 2 apoptosis related cysteine peptidase
    • ICE
    • ICH 1 protease
    • ICH 1L
    • ICH 1L/1S
    • ICH1
    • IL 1 beta converting enzyme
    • IL1BC
    • Interleukin 1 B converting enzyme
    • Interleukin 1 beta convertase
    • Interleukin 1 beta converting enzyme
    • NEDD 2
    • NEDD2
    • NEDD2 apoptosis regulatory
    • Neural precursor cell expressed developmentally down regulated 2
    • Neural precursor cell expressed developmentally down regulated protein 2
    • P45
    • Protease ICH 1
    see all

Protocols

References

ab102487 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for contacting us with your questions.

We do have some panels for caspase activity measurement:

1) Ab102486: Includes substrates for caspases 1,2,3,5,6,8, and 9. It does not include substrates for caspases 4 and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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