Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Caspr antibody [EPR7828] (ab133634)

Knockout Tested Rabbit recombinant monoclonal Caspr antibody [EPR7828]. Validated in WB, IHC and tested in Mouse, Rat, Human. Cited in 1 publication(s).

Overview

  • Product name

    Anti-Caspr antibody [EPR7828]
    See all Caspr primary antibodies
  • Description

    Rabbit monoclonal [EPR7828] to Caspr
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Caspr aa 700-800 (extracellular). The exact sequence is proprietary.

  • Positive control

    • C6 cell lysate, Neuro2a cell lysate, U87 MG cell lysate, Fetal brain lysate, Fetal lung lysate, Human glioma tissue. ICC/IF: Neuro-2a cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.40 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7828
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa).
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol
ICC/IF 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.
    • Tissue specificity

      Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis.
    • Sequence similarities

      Belongs to the neurexin family.
      Contains 2 EGF-like domains.
      Contains 1 F5/8 type C domain.
      Contains 1 fibrinogen C-terminal domain.
      Contains 4 laminin G-like domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Caspr antibody
      • Caspr1 antibody
      • CNTNAP antibody
      • Cntnap1 antibody
      • CNTP1_HUMAN antibody
      • Contactin associated protein 1 antibody
      • Contactin-associated protein 1 antibody
      • MHDNIV antibody
      • NCP1 antibody
      • Neurexin 4 antibody
      • Neurexin IV antibody
      • Neurexin-4 antibody
      • Nrxn4 antibody
      • p190 antibody
      • Paranodin antibody
      see all

    Images

    • All lanes : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : CNTNAP1 (Caspr) knockout HAP1 whole cell lysate
      Lane 3 : MEF whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 156 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab133634 observed at 175 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab133634 was shown to recognize Caspr in wild-type HAP1 cells as signal was lost at the expected MW in CNTNAP1 (Caspr) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CNTNAP1 (Caspr) knockout samples were subjected to SDS-PAGE. ab133634 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at  1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Caspr with purified ab133634 at 1/100 (5.7 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemical analysis of paraffin embedded Human glioma tissue labelling Caspr with ab133634 at 1/100.
    • All lanes : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Neuro2a cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 156 kDa
      Observed band size: 175 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zhang SH  et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). Read more (PubMed: 30792309) »
    • Zhao WD  et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun 9:2296 (2018). Read more (PubMed: 29895952) »
    See all 2 Publications for this product

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