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    caspr-antibody-epr7828-ab133634.pdf

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Caspr antibody [EPR7828] (ab133634)

  • Datasheet
Reviews (4) Submit a question References (2)

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Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)
  • Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [EPR7828] (ab133634)
  • Anti-Caspr antibody [EPR7828] (ab133634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7828] to Caspr
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Caspr antibody [EPR7828]
    See all Caspr primary antibodies
  • Description

    Rabbit monoclonal [EPR7828] to Caspr
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Caspr aa 700-800 (extracellular). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa (Boiled and Unboiled), SH-SY5Y (Boiled and Unboiled), Neuro-2a (Boiled and Unboiled), PC-12 (Boiled and Unboiled) IHC-P: Human cerebrum and Human astrocytoma tissue sections ICC/IF: Neuro-2a cells
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.40 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7828
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Caspr antibody [EPR7828] - BSA and Azide free (ab248583)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa).
IHC-P (4)
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. 

ICC/IF
1/100.
Notes
WB
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. 

ICC/IF
1/100.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.
  • Tissue specificity

    Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis.
  • Sequence similarities

    Belongs to the neurexin family.
    Contains 2 EGF-like domains.
    Contains 1 F5/8 type C domain.
    Contains 1 fibrinogen C-terminal domain.
    Contains 4 laminin G-like domains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P78357 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8506 Human
    • Entrez Gene: 53321 Mouse
    • Entrez Gene: 84008 Rat
    • Omim: 602346 Human
    • SwissProt: P78357 Human
    • SwissProt: O54991 Mouse
    • SwissProt: P97846 Rat
    • Unigene: 408730 Human
    • Unigene: 474527 Mouse
    • Unigene: 88654 Rat
    see all
  • Alternative names

    • Caspr antibody
    • Caspr1 antibody
    • CNTNAP antibody
    • Cntnap1 antibody
    • CNTP1_HUMAN antibody
    • Contactin associated protein 1 antibody
    • Contactin-associated protein 1 antibody
    • MHDNIV antibody
    • NCP1 antibody
    • Neurexin 4 antibody
    • Neurexin IV antibody
    • Neurexin-4 antibody
    • Nrxn4 antibody
    • p190 antibody
    • Paranodin antibody
    see all

Images

  • Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    Lanes 1-7 : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution (Purified)
    Lane 8 : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate boiled
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled
    Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate boiled
    Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate unboiled
    Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate boiled
    Lane 6 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate unboiled
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate boiled
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate unboiled

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 156 kDa
    Observed band size: 220 kDa why is the actual band size different from the predicted?



    The molecular weight is consistent with that has been described in the literature (PMID: 20610764).

    We are unsure about the nature of extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Caspr with Purified ab133634 at 1:2000 dilution (0.29 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [EPR7828] (ab133634)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Caspr with Purified ab133634 at 1:2000 dilution (0.29 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    Western blot - Anti-Caspr antibody [EPR7828] (ab133634)
    All lanes : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CNTNAP1 (Caspr) knockout HAP1 whole cell lysate
    Lane 3 : MEF whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 156 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab133634 observed at 175 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab133634 was shown to recognize Caspr in wild-type HAP1 cells as signal was lost at the expected MW in CNTNAP1 (Caspr) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CNTNAP1 (Caspr) knockout samples were subjected to SDS-PAGE. ab133634 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at  1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [EPR7828] (ab133634)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [EPR7828] (ab133634)

    Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Caspr with purified ab133634 at 1/100 (5.7 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Caspr antibody [EPR7828] (ab133634)
    Anti-Caspr antibody [EPR7828] (ab133634)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab133634? Please let us know so that we can cite the reference in this datasheet.

ab133634 has been referenced in 2 publications.

  • Zhang SH  et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). PubMed: 30792309
  • Zhao WD  et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun 9:2296 (2018). PubMed: 29895952

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr antibody [EPR7828]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (cerebrum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
cerebrum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Oct 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr antibody [EPR7828]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Cerebellum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Oct 27 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr antibody [EPR7828]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Cerebellum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Sep 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr antibody [EPR7828]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Sep 01 2019

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