Recombinant Anti-Caspr antibody [EPR7828] (ab133634)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7828] to Caspr
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Caspr antibody [EPR7828]
See all Caspr primary antibodies -
Description
Rabbit monoclonal [EPR7828] to Caspr -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Caspr aa 700-800 (extracellular). The exact sequence is proprietary.
-
Positive control
- WB: HeLa (Boiled and Unboiled), SH-SY5Y (Boiled and Unboiled), Neuro-2a (Boiled and Unboiled), PC-12 (Boiled and Unboiled) IHC-P: Human cerebrum and Human astrocytoma tissue sections ICC/IF: Neuro-2a cells
-
General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7828 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa).
|
|
IHC-P | (4) |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ICC/IF |
1/100.
|
Notes |
---|
WB
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ICC/IF
1/100. |
Target
-
Function
Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells. -
Tissue specificity
Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis. -
Sequence similarities
Belongs to the neurexin family.
Contains 2 EGF-like domains.
Contains 1 F5/8 type C domain.
Contains 1 fibrinogen C-terminal domain.
Contains 4 laminin G-like domains. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 8506 Human
- Entrez Gene: 53321 Mouse
- Entrez Gene: 84008 Rat
- Omim: 602346 Human
- SwissProt: P78357 Human
- SwissProt: O54991 Mouse
- SwissProt: P97846 Rat
- Unigene: 408730 Human
see all -
Alternative names
- Caspr antibody
- Caspr1 antibody
- CNTNAP antibody
see all
Images
-
Lanes 1-7 : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution (Purified)
Lane 8 : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate boiled
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate boiled
Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate unboiled
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate boiled
Lane 6 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate unboiled
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate boiled
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 156 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?The molecular weight is consistent with that has been described in the literature (PMID: 20610764).
We are unsure about the nature of extra bands.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Caspr with Purified ab133634 at 1:2000 dilution (0.29 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Caspr with Purified ab133634 at 1:2000 dilution (0.29 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
-
All lanes : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CNTNAP1 (Caspr) knockout HAP1 whole cell lysate
Lane 3 : MEF whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 156 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133634 observed at 175 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab133634 was shown to recognize Caspr in wild-type HAP1 cells as signal was lost at the expected MW in CNTNAP1 (Caspr) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CNTNAP1 (Caspr) knockout samples were subjected to SDS-PAGE. ab133634 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Caspr with purified ab133634 at 1/100 (5.7 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab133634 has been referenced in 3 publications.
- Zhao N et al. Clinical features of Guillain-Barré syndrome with anti-neurofascin 155 antibody. Acta Neurol Scand 146:553-561 (2022). PubMed: 36237129
- Zhang SH et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). PubMed: 30792309
- Zhao WD et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun 9:2296 (2018). PubMed: 29895952