Anti-Caspr2/CNTNAP2 antibody (ab153856)
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Overview
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Product name
Anti-Caspr2/CNTNAP2 antibody
See all Caspr2/CNTNAP2 primary antibodies -
Description
Rabbit polyclonal to Caspr2/CNTNAP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Caspr2/CNTNAP2 aa 287-633.
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Positive control
- WB: Molt-4, and mouse brain lysates; ICC/IF: HeLa cells and DIV14 rat E18 primary cortical neurons . IHC-Fr: E13.5 Rat brain and embryonic mouse brain (mE18.5).
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General notes
This product was previously labelled as Caspr2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab153856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 148 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-Fr | 1/250. |
Target
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Function
May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. -
Tissue specificity
Predominantly expressed in nervous system. -
Involvement in disease
Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2. -
Sequence similarities
Belongs to the neurexin family.
Contains 2 EGF-like domains.
Contains 1 F5/8 type C domain.
Contains 1 fibrinogen C-terminal domain.
Contains 4 laminin G-like domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 26047 Human
- Entrez Gene: 66797 Mouse
- Omim: 604569 Human
- SwissProt: Q9UHC6 Human
- SwissProt: Q9CPW0 Mouse
- Unigene: 655684 Human
- Unigene: 440084 Mouse
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Alternative names
- AUTS15 antibody
- CDFE antibody
- Cell recognition molecule Caspr2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min staining Caspr2/CNTNAP2 protein by immunofluorescent analysis (1:500) (green).
Counter stained using beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500 (red) and DAPI (blue). -
Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)Frozen section of embryonic mouse brain (mE18.5) staining Caspr2/CNTNAP2 proteins in embryonic mouse brain by immunohistochemical analysis.
Red: ab153856 diluted at 1:250.
Blue: DAPI. -
Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)Frozen sectioned E13.5 Rat brain staining Caspr2/CNTNAP2 protein expression by immunohistochemical analysis.
Green: ab153856 diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500.
Blue: Fluoroshield with DAPI. -
Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/5000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 148 kDa
5% SDS PAGE -
Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 148 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Caspr2/CNTNAP2 with ab153856 at 1/200 dilution. Lower panel is merged with DNA probe.
References
ab153856 has not yet been referenced specifically in any publications.
