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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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Anti-Caspr2/CNTNAP2 antibody (ab153856)

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Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)
  • Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)
  • Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)
  • Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)
  • Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)

Key features and details

  • Rabbit polyclonal to Caspr2/CNTNAP2
  • Suitable for: WB, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Caspr2/CNTNAP2 antibody
    See all Caspr2/CNTNAP2 primary antibodies
  • Description

    Rabbit polyclonal to Caspr2/CNTNAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Caspr2/CNTNAP2 aa 287-633.

  • Positive control

    • WB: Molt-4, and mouse brain lysates; ICC/IF: HeLa cells and DIV14 rat E18 primary cortical neurons . IHC-Fr: E13.5 Rat brain and embryonic mouse brain (mE18.5).
  • General notes

     This product was previously labelled as Caspr2

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Mouse brain tissue lysate - total protein (ab30151)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 148 kDa.
ICC/IF
1/100 - 1/1000.
IHC-Fr
1/250.
Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 148 kDa.
ICC/IF
1/100 - 1/1000.
IHC-Fr
1/250.

Target

  • Function

    May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.
  • Tissue specificity

    Predominantly expressed in nervous system.
  • Involvement in disease

    Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
    Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
    Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.
  • Sequence similarities

    Belongs to the neurexin family.
    Contains 2 EGF-like domains.
    Contains 1 F5/8 type C domain.
    Contains 1 fibrinogen C-terminal domain.
    Contains 4 laminin G-like domains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9UHC6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 26047 Human
    • Entrez Gene: 66797 Mouse
    • Omim: 604569 Human
    • SwissProt: Q9UHC6 Human
    • SwissProt: Q9CPW0 Mouse
    • Unigene: 655684 Human
    • Unigene: 440084 Mouse
    • Alternative names

      • AUTS15 antibody
      • CDFE antibody
      • Cell recognition molecule Caspr2 antibody
      • CNTNAP2 antibody
      • CNTP2 antibody
      • CNTP2_HUMAN antibody
      • Contactin-associated protein-like 2 antibody
      • Homolog of Drosophila neurexin IV antibody
      • NRXN4 antibody
      • PTHSL1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)

      DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min staining Caspr2/CNTNAP2 protein by immunofluorescent analysis (1:500) (green).
      Counter stained using beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500 (red) and DAPI (blue).

    • Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)

      Frozen section of embryonic mouse brain (mE18.5) staining Caspr2/CNTNAP2 proteins in embryonic mouse brain by immunohistochemical analysis. 
      Red: ab153856 diluted at 1:250. 
      Blue: DAPI.

    • Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)

      Frozen sectioned E13.5 Rat brain staining Caspr2/CNTNAP2 protein expression by immunohistochemical analysis.
      Green: ab153856 diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500.
      Blue: Fluoroshield with DAPI.

    • Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/5000 dilution + Molt-4 whole cell lysate at 30 µg

      Predicted band size: 148 kDa



      5% SDS PAGE
    • Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

      Predicted band size: 148 kDa



      5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)

      Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Caspr2/CNTNAP2 with ab153856 at 1/200 dilution. Lower panel is merged with DNA probe.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab153856? Please let us know so that we can cite the reference in this datasheet.

    ab153856 has not yet been referenced specifically in any publications.

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