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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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RecombinantRabMAb

Recombinant Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

  • Datasheet
Reviews (3) Submit a question References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
  • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
  • Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8738] to Caspr2/CNTNAP2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Caspr2/CNTNAP2 antibody [EPR8738]
    See all Caspr2/CNTNAP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8738] to Caspr2/CNTNAP2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human Caspr2/CNTNAP2 aa 1300-1400 (C terminal).

  • Positive control

    • Fetal brain and Human glioma lysates; Human brain tissue.
  • General notes

     

     

     This product was previously labelled as Caspr2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8738
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker

Associated products

  • Alternative Versions

    • Anti-Caspr2/CNTNAP2 antibody [EPR8738] - BSA and Azide free (ab248788)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).
IHC-P (3)
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.
    • Tissue specificity

      Predominantly expressed in nervous system.
    • Involvement in disease

      Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
      Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
      Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.
    • Sequence similarities

      Belongs to the neurexin family.
      Contains 2 EGF-like domains.
      Contains 1 F5/8 type C domain.
      Contains 1 fibrinogen C-terminal domain.
      Contains 4 laminin G-like domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q9UHC6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 26047 Human
      • Entrez Gene: 66797 Mouse
      • Omim: 604569 Human
      • SwissProt: Q9UHC6 Human
      • SwissProt: Q9CPW0 Mouse
      • Unigene: 655684 Human
      • Unigene: 440084 Mouse
      • Alternative names

        • AUTS15 antibody
        • CDFE antibody
        • Cell recognition molecule Caspr2 antibody
        • CNTNAP2 antibody
        • CNTP2 antibody
        • CNTP2_HUMAN antibody
        • Contactin-associated protein-like 2 antibody
        • Homolog of Drosophila neurexin IV antibody
        • NRXN4 antibody
        • PTHSL1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

        Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab137052 at a working dilution of 1 in 2000. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

        Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Caspr2/CNTNAP2 with unpurified ab137052 at dilution 1/250.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (purified) + Human glioma tissue lysate at 20 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa
        why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        All lanes : Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (unpurified)

        Lane 1 : Fetal brain lysate
        Lane 2 : Human glioma lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?

      • Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab137052? Please let us know so that we can cite the reference in this datasheet.

      ab137052 has been referenced in 2 publications.

      • Giannoccaro MP  et al. Behaviour and neuropathology in mice injected with human contactin-associated protein 2 antibodies. Brain 142:2000-2012 (2019). PubMed: 31079141
      • Patterson KR  et al. Mechanisms of Caspr2 antibodies in autoimmune encephalitis and neuromyotonia. Ann Neurol 83:40-51 (2018). PubMed: 29244234

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr2/CNTNAP2 antibody [EPR8738]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Sep 10 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr2/CNTNAP2 antibody [EPR8738]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Sep 10 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr2 antibody [EPR8738]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Cultured Cells (HEK 293 cells)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Specification
      HEK 293 cells
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Nov 13 2018

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