Recombinant
RabMAb

Recombinant Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

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Overview

  • Product name

    Anti-Caspr2/CNTNAP2 antibody [EPR8738]
    See all Caspr2/CNTNAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8738] to Caspr2/CNTNAP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human Caspr2/CNTNAP2 aa 1300-1400 (C terminal).

  • Positive control

    • Fetal brain and Human glioma lysates; Human brain tissue.

  • General notes

     

     

     This product was previously labelled as Caspr2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8738

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.

    • Tissue specificity

      Predominantly expressed in nervous system.

    • Involvement in disease

      Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
      Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
      Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.

    • Sequence similarities

      Belongs to the neurexin family.
      Contains 2 EGF-like domains.
      Contains 1 F5/8 type C domain.
      Contains 1 fibrinogen C-terminal domain.
      Contains 4 laminin G-like domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • AUTS15 antibody
        • CDFE antibody
        • Cell recognition molecule Caspr2 antibody
        • CNTNAP2 antibody
        • CNTP2 antibody
        • CNTP2_HUMAN antibody
        • Contactin-associated protein-like 2 antibody
        • Homolog of Drosophila neurexin IV antibody
        • NRXN4 antibody
        • PTHSL1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

        Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab137052 at a working dilution of 1 in 2000. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)

        Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Caspr2/CNTNAP2 with unpurified ab137052 at dilution 1/250.

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (purified) + Human glioma tissue lysate at 20 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa
        why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg

        Secondary
        HRP goat anti-rabbit (H+L) at 1/1000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?



        Blocking buffer: 5% NFDM/TBST

        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        Western blot - Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052)
        All lanes : Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (unpurified)

        Lane 1 : Fetal brain lysate
        Lane 2 : Human glioma lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

        Predicted band size: 148 kDa
        Observed band size: 152 kDa why is the actual band size different from the predicted?

      References

      This product has been referenced in:

      See 1 Publication for this product

      Publishing research using ab137052? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspr2 antibody [EPR8738]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Cultured Cells (HEK 293 cells)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Specification
      HEK 293 cells
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Nov 13 2018

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