Anti-CaSR antibody (ab137408)
Key features and details
- Rabbit polyclonal to CaSR
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CaSR antibody
See all CaSR primary antibodies -
Description
Rabbit polyclonal to CaSR -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cat, Dog
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Immunogen
Recombinant fragment corresponding to Human CaSR aa 151-376.
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Positive control
- HeLa whole cell lysate (ab150035); HeLa cells; SAS xenograft tissue; 293T, A431 and H1299.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| IHC-P | (1) |
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 121 kDa.
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| Notes |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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WB
1/500 - 1/3000. Predicted molecular weight: 121 kDa. |
Target
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Function
Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. -
Involvement in disease
Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Post-translational
modificationsN-glycosylated.
Ubiquitinated by RNF19A; which induces proteasomal degradation. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100302732 Cat
- Entrez Gene: 281038 Cow
- Entrez Gene: 846 Human
- Entrez Gene: 12374 Mouse
- Entrez Gene: 24247 Rat
- Omim: 601199 Human
- SwissProt: P35384 Cow
- SwissProt: P41180 Human
see all -
Alternative names
- Ca sensing receptor antibody
- Ca2+ sensing receptor 1 antibody
- Ca2+ sensing receptor antibody
see all
Images
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Western blot - Anti-CaSR antibody (ab137408)Anti-CaSR antibody (ab137408) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 121 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody (ab137408)Immunohistochemical analysis of paraffin-embedded SAS xenograft labelling CaSR with ab137408 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody (ab137408)This image is courtesy of an anonymous Abreviewab137408 staining CaSR in Human colon cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with protein block serum-free for 20 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 2 hours at 22°C. A HRP-conjugated Goat polyclonal was used as the 0secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (ab137408)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling CaSR with ab137408 at 1/200 dilution. Lower panel merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab137408 has been referenced in 7 publications.
- Hui Q et al. Molecular distribution and localization of extracellular calcium-sensing receptor (CaSR) and vitamin D receptor (VDR) at three different laying stages in laying hens (Gallus gallus domesticus). Poult Sci 100:101060 (2021). PubMed: 33752067
- Yuan H et al. Calcium sensing receptor involving in therapy of embryonic stem cell transplantation alleviates acute myocardial infarction by inhibiting apoptosis and oxidative stress in rats. Iran J Basic Med Sci 23:1353-1359 (2020). PubMed: 33149870
- Ramezani A et al. Efficient detection of eukaryotic calcium-sensing receptor (CaSR) by polyclonal antibody against prokaryotic expressed truncated CaSR. Mol Biol Rep 47:7723-7734 (2020). PubMed: 33001312
- Yang W et al. Calcium intake and risk of colorectal cancer according to expression status of calcium-sensing receptor (CASR). Gut 67:1475-1483 (2018). PubMed: 28676564
- Momen-Heravi F et al. Tumor expression of calcium sensing receptor and colorectal cancer survival: Results from the nurses' health study and health professionals follow-up study. Int J Cancer 141:2471-2479 (2017). PubMed: 28856682
- Ahearn TU et al. Calcium-Sensing Receptor Tumor Expression and Lethal Prostate Cancer Progression. J Clin Endocrinol Metab 101:2520-7 (2016). PubMed: 27115058
- Xie J et al. Calcium-sensing receptor is involved in the pathogenesis of fat emulsion-induced insulin resistance in rats. Mol Med Rep 12:2043-8 (2015). PubMed: 25892159