Overview

  • Product name

  • Description

    Rabbit polyclonal to CaSR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Rat CaSR aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab18608)

  • Positive control

    • WB: CASR 293T cell transient overexpression cell lysates.
  • General notes

     This product was previously labelled as Calcium Sensing Receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab18200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 120-130 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • Involvement in disease

    Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications

    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • Calcium sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCaR1 antibody
    see all

Images

  • All lanes : Anti-CaSR antibody (ab18200) at 1 µg/ml

    Lane 1 : CaSR 293T Cell Transient Overexpression Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size: 130 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 200 kDa, 50 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    In this experiment Hek293 was used as a negative control.

  • ab18200 staining CaSR in rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde. Samples were incubated with primary antibody (1/100 in PBST) for 24 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    This antibody gave a weak staining using direct fluorescence, using amplification (secondary biotinylated) produced nice staining. Image taken in the hypothalamic paraventricular nucleus, the staining is not located on the cell membrane but rather on the cytoplasm or nucleus of the cells. B is a higher power magnification of A. The pictures were taken using objectives X10 and X40.

    See Abreview

  • Lane 1 = HEK cell lysate used as negative control. Lane 2 = Post nuclear fraction of rat brain (P21). As secondary goat anti-rabbit HRP conjugated was used. Please see accompanying abreview for additional information

    See Abreview

References

This product has been referenced in:

  • Zhang X  et al. Mechanisms of Pancreatic Injury Induced by Basic Amino Acids Differ Between L-Arginine, L-Ornithine, and L-Histidine. Front Physiol 9:1922 (2018). Read more (PubMed: 30697165) »
  • Ahmad F  et al. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue. Sci Rep 6:28056 (2016). Read more (PubMed: 27321128) »
See all 5 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Human vascular smooth muscle cell)
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
10 µg
Specification
Human vascular smooth muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 29 2016

Question
Answer

Thank you for calling Abcam earlier today.
I am sorry that you were experiencing issues with ab18200 in western blot. As we discussed, I am sending you ab79038 as free of charge replacement. Your new order number is *.
If there is anything else I can help you with, or if you experience any issues with the new antibody, then please let me know.

Read More

Question
Answer

Thank you for contacting us.

The catalogue number of blocking peptide is ab18608.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain sections)
Permeabilization
No
Specification
brain sections
Fixative
Paraformaldehyde

Dr. Sophie Pezet

Verified customer

Submitted Jul 25 2008

Application
Western blot
Sample
Rat Cell lysate - other (post nuclear extract of rat brain)
Gel Running Conditions
Reduced Denaturing
Specification
post nuclear extract of rat brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C

Dr. Sriharsha Kantamneni

Verified customer

Submitted Oct 31 2007

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