Key features and details
- Rabbit polyclonal to CaSR
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-CaSR antibody
See all CaSR primary antibodies
DescriptionRabbit polyclonal to CaSR
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Rat CaSR aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as
This product was previously labelled as Calcium Sensing Receptor
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab18200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 120-130 kDa.|
FunctionSenses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
Tissue specificityExpressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
Involvement in diseaseDefects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Ubiquitinated by RNF19A; which induces proteasomal degradation.
Cellular localizationCell membrane.
- Information by UniProt
- Ca sensing receptor antibody
- Ca2+ sensing receptor 1 antibody
- Ca2+ sensing receptor antibody
All lanes : Anti-CaSR antibody (ab18200) at 1 µg/ml
Lane 1 : CaSR 293T Cell Transient Overexpression Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 50 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
In this experiment Hek293 was used as a negative control.
Lane 1 = HEK cell lysate used as negative control. Lane 2 = Post nuclear fraction of rat brain (P21). As secondary goat anti-rabbit HRP conjugated was used. Please see accompanying abreview for additional information
ab18200 has been referenced in 5 publications.
- Zhang X et al. Mechanisms of Pancreatic Injury Induced by Basic Amino Acids Differ Between L-Arginine, L-Ornithine, and L-Histidine. Front Physiol 9:1922 (2018). PubMed: 30697165
- Ahmad F et al. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue. Sci Rep 6:28056 (2016). PubMed: 27321128
- Park YH et al. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol 17:914-21 (2016). PubMed: 27270401
- Rizzardi AE et al. Evaluation of protein biomarkers of prostate cancer aggressiveness. BMC Cancer 14:244 (2014). PubMed: 24708576
- Lee GS et al. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature 492:123-7 (2012). WB . PubMed: 23143333