Product nameAnti-CaSR antibody
See all CaSR primary antibodies
DescriptionRabbit polyclonal to CaSR
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human CaSR aa 1-100 (N terminal). The exact sequence is proprietary. (NP_000379.2).
Database link: P41180
- WB: Rat kidney lysate. ICC/IF: SK-N-BE cells.
This product was previously labelled as Calcium Sensing Receptor
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).|
FunctionSenses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
Tissue specificityExpressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
Involvement in diseaseDefects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Ubiquitinated by RNF19A; which induces proteasomal degradation.
Cellular localizationCell membrane.
- Information by UniProt
- Ca sensing receptor antibody
- Ca2+ sensing receptor 1 antibody
- Ca2+ sensing receptor antibody
4% formaldehyde-fixed SK-N-BE cells stained for CaSR (green) using ab223360 at 1/100 dilution for 60 min at room temperature in ICC/IF.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223360 (D) Merge.
Anti-CaSR antibody (ab223360) at 1/1000 dilution + Rat kidney lysate at 15 µg
Goat Anti-Rabbit IgG HRP at 1/200 dilution
Predicted band size: 121 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% skim milk in TBST.
Primary incubation: 16 hours at 4ºC.
Color Development: TMB.
ab223360 has not yet been referenced specifically in any publications.