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  1. Link

    casr-antibody-ab223360.pdf

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Anti-CaSR antibody (ab223360)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (ab223360)
  • Western blot - Anti-CaSR antibody (ab223360)

Key features and details

  • Rabbit polyclonal to CaSR
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
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Recombinant Human CaSR protein (ab114274)
Primary
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Anti-CaSR (phospho T888) antibody (ab182619)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CaSR antibody
    See all CaSR primary antibodies
  • Description

    Rabbit polyclonal to CaSR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human CaSR aa 1-100 (N terminal). The exact sequence is proprietary. (NP_000379.2).
    Database link: P41180

  • Positive control

    • WB: Rat kidney lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CaSR protein (ab114274)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
ICC/IF
1/100.
Notes
WB
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
ICC/IF
1/100.

Target

  • Function

    Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • Involvement in disease

    Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications

    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41180 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 846 Human
    • Entrez Gene: 24247 Rat
    • Omim: 601199 Human
    • SwissProt: P41180 Human
    • SwissProt: P48442 Rat
    • Unigene: 435615 Human
    • Unigene: 10019 Rat
    • Alternative names

      • Ca sensing receptor antibody
      • Ca2+ sensing receptor 1 antibody
      • Ca2+ sensing receptor antibody
      • Calcium sensing receptor antibody
      • CAR antibody
      • CaSR antibody
      • CASR_HUMAN antibody
      • EIG8 antibody
      • Extracellular calcium sensing receptor antibody
      • Extracellular calcium sensing receptor [Precursor] antibody
      • Extracellular calcium-sensing receptor antibody
      • FHH antibody
      • FIH antibody
      • GPRC2A antibody
      • HHC antibody
      • HHC1 antibody
      • Hypocalciuric hypercalcemia 1 antibody
      • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
      • MGC138441 antibody
      • NSHPT antibody
      • Parathyroid Ca(2+) sensing receptor 1 antibody
      • Parathyroid Cell calcium sensing receptor antibody
      • Parathyroid Cell calcium-sensing receptor antibody
      • PCAR 1 antibody
      • PCaR1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (ab223360)
      Immunocytochemistry/ Immunofluorescence - Anti-CaSR antibody (ab223360)

      4% formaldehyde-fixed SK-N-BE cells stained for CaSR (green) using ab223360 at 1/100 dilution for 60 min at room temperature in ICC/IF.

      (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223360 (D) Merge.

       

    • Western blot - Anti-CaSR antibody (ab223360)
      Western blot - Anti-CaSR antibody (ab223360)
      Anti-CaSR antibody (ab223360) at 1/1000 dilution + Rat kidney lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG HRP at 1/200 dilution

      Predicted band size: 121 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% skim milk in TBST.

      Primary incubation: 16 hours at 4ºC.

      Color Development: TMB.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab223360? Please let us know so that we can cite the reference in this datasheet.

    ab223360 has been referenced in 1 publication.

    • Zhao Y  et al. Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin-angiotensin system. Exp Ther Med 20:818-829 (2020). PubMed: 32742325

    Customer reviews and Q&As

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