Overview

  • Product name

  • Description

    Rabbit polyclonal to CaSR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human CaSR aa 1-100 (N terminal). The exact sequence is proprietary. (NP_000379.2).
    Database link: P41180

  • Positive control

    • WB: Rat kidney lysate. ICC/IF: SK-N-BE cells.
  • General notes

     This product was previously labelled as Calcium Sensing Receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab223360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
ICC/IF 1/100.

Target

  • Function

    Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • Involvement in disease

    Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications

    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • Calcium sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCaR1 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for CaSR (green) using ab223360 at 1/100 dilution for 60 min at room temperature in ICC/IF.

    (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223360 (D) Merge.

     

  • Anti-CaSR antibody (ab223360) at 1/1000 dilution + Rat kidney lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/200 dilution

    Predicted band size: 121 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% skim milk in TBST.

    Primary incubation: 16 hours at 4ºC.

    Color Development: TMB.

References

ab223360 has not yet been referenced specifically in any publications.

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