Overview

  • Product name

  • Description

    Rabbit polyclonal to CaSR
  • Host species

    Rabbit
  • Specificity

    Does not cross react to other calcium binding proteins.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to CaSR aa 47-69 (N terminal).
    Sequence:

    KDQDLKSRPESVECIRYNFRGFR

  • General notes

     This product was previously labelled as Calcium Sensing Receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab79829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
ELISA 1/10000.

Target

  • Function

    Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • Involvement in disease

    Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications

    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • Calcium sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCaR1 antibody
    see all

References

ab79829 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. The amino acid 374 -391 falls more towards N-terminal end of the protein; this sequence is present in the extracellular domain which extends from amino acid 20 to 612 (http://www.uniprot.org/uniprot/P41180). The sequence homology shows the suitability of 6 antibodies ab19347 ab27493 ab31212 ab79038 ab18200 ab79829    Unfortunately none of these antibodies are tested in Flow Cytometry. However this does not mean the antibodies are not suitable for FLow Cytometry this only mean we do not have tested data to confirm the positive or negative results.   Even ab19347 is not tested in flow Cytometry so I would suggest using this antibody in Flow Cytometry as normal. We are happy in providing help with troubleshooting if the antibody fails. In case you are interested in buying new anti Calcium sensing receptor antibody; I can check if the purchase is eligible for Abreview testing discount programme. You have to provide us the cell line details; we will then create a discount code for you. The discount code will be active once you use the antibody in Flow Cytomery and submit an Abreview. The code will give you next antibody free of charge in exchange of a Flow Cytometry positive or negative Abreview. Contact me for discount code if you are interested in this offer. Please check our website at the following link for compatible secondary antibodies; https://www.abcam.com/index.html?pageconfig=productmap&cl=918 https://www.abcam.com/index.html?pageconfig=searchresults ab79091 Rat monoclonal [SB84a] Secondary Antibody to Mouse IgG2a - gamma chain (ab79091) I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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