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    casr-antibody-hl1499-bsa-and-azide-free-ab264481.pdf

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Recombinant

Recombinant Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)
  • Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [HL1499] to CaSR - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CaSR antibody [HL1499] - BSA and Azide free
    See all CaSR primary antibodies
  • Description

    Mouse monoclonal [HL1499] to CaSR - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr or WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Mouse and rat kidney tissue.
  • General notes

    ab264481 is the carrier-free version of ab255957.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HL1499
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CaSR antibody [HL1499] (ab255957)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab264481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 0.665 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 0.665 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IHC-Fr or WB.

Target

  • Function

    Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • Involvement in disease

    Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications

    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41180 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 846 Human
    • Entrez Gene: 12374 Mouse
    • Entrez Gene: 24247 Rat
    • Omim: 601199 Human
    • SwissProt: P41180 Human
    • SwissProt: Q9QY96 Mouse
    • SwissProt: P48442 Rat
    • Unigene: 435615 Human
    • Unigene: 103619 Mouse
    • Unigene: 10019 Rat
    see all
  • Alternative names

    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • Calcium sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCaR1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CaSR with ab255957 at 0.665µg/ml, followed by ready to use secondary. Positive staining on the ascending limb in rat kidney is observed. The section was incubated with ab255957 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab255957).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CaSR with ab255957 at 0.665µg/ml, followed by ready to use secondary. Positive staining on the ascending limb in mouse kidney is observed. The section was incubated with ab255957 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab255957).

     

  • Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)
    Anti-CaSR antibody [HL1499] - BSA and Azide free (ab264481)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab264481? Please let us know so that we can cite the reference in this datasheet.

ab264481 has not yet been referenced specifically in any publications.

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