Anti-CaSR antibody - N-terminal (ab219182)
Key features and details
- Rabbit polyclonal to CaSR - N-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CaSR antibody - N-terminal
See all CaSR primary antibodies -
Description
Rabbit polyclonal to CaSR - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Xenopus laevis, Monkey, Xenopus tropicalis, Bat
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Immunogen
Synthetic peptide within Human CaSR (N terminal). The exact sequence is proprietary. 17 amino acid peptide from N-terminal extracellular domain.
Database link: P41180 -
Positive control
- IHC-P: Human thyroid medullary carcinoma, kidney and parathyroid tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. -
Involvement in disease
Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Post-translational
modificationsN-glycosylated.
Ubiquitinated by RNF19A; which induces proteasomal degradation. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 281038 Cow
- Entrez Gene: 846 Human
- Entrez Gene: 12374 Mouse
- Entrez Gene: 24247 Rat
- Omim: 601199 Human
- SwissProt: P35384 Cow
- SwissProt: P41180 Human
- SwissProt: Q9QY96 Mouse
see all -
Alternative names
- Ca sensing receptor antibody
- Ca2+ sensing receptor 1 antibody
- Ca2+ sensing receptor antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody - N-terminal (ab219182)
Immunohistochemical analysis of paraffin embedded formalin fixed human thyroid, medullary carcinoma tissue labeling CaSR using ab219182 at 8 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody - N-terminal (ab219182)Immunohistochemical analysis of paraffin embedded formalin fixed human kidney, diabetes tissue labeling CaSR using ab219182 at 8 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody - N-terminal (ab219182)Immunohistochemical analysis of paraffin embedded formalin fixed human parathyroid tissue labeling CaSR using ab219182 at 8 µg/mL.
Datasheets and documents
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Datasheet download
References (0)
ab219182 has not yet been referenced specifically in any publications.
