Anti-CAST antibody (ab75252)
Key features and details
- Rabbit polyclonal to CAST
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CAST antibody
See all CAST primary antibodies -
Description
Rabbit polyclonal to CAST -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CAST aa 200-300. NP_036231.1
Database link: O15446-1 -
Positive control
- HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75252 was affinity purified using an epitope specific to CAST immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. -
Sequence similarities
Belongs to the eukaryotic RPA34 RNA polymerase subunit family. -
Post-translational
modificationsIsoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 10849 Human
- Omim: 107325 Human
- SwissProt: O15446 Human
- Unigene: 710495 Human
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Alternative names
- A34.5 antibody
- Anti sense to ERCC 1 protein antibody
- Antisense to ERCC-1 protein antibody
see all
Images
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CAST was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab75252 at 3 µg per reaction. Western blot was performed on the immunoprecipitate using ab70497 at 1 µg/mL.
Lane 1: rabbit anti-PAF49 antibody IP in HeLa whole cell lysate.
Lane 2: ab75252 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling CAST with ab75252 at 1/5000 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab75252 has not yet been referenced specifically in any publications.