Key features and details
- Rabbit polyclonal to CAST
- Suitable for: WB, IP, IHC-P, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CAST antibody
See all CAST primary antibodies
DescriptionRabbit polyclonal to CAST
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to Human CAST. corresponding to a region between residues 404 and 454
Database link: NP_036231.1
- HeLa whole cell lysate.
Previously labelled as PAF49.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab76933 was affinity purified using an epitope specific to CAST immobilized on solid support.
Our Abpromise guarantee covers the use of ab76933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 55 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC||1/50 - 1/250.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
|ICC/IF||1/50 - 1/250.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Sequence similaritiesBelongs to the eukaryotic RPA34 RNA polymerase subunit family.
modificationsIsoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
- Information by UniProt
- A34.5 antibody
- Anti sense to ERCC 1 protein antibody
- Antisense to ERCC-1 protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAST with ab76933 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-CAST antibody (ab76933) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 55 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
ab76933 at 1µg/ml staining CAST in HeLa whole cell lysate immunoprecipitated using ab76933 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
ab76933 has not yet been referenced specifically in any publications.