Overview

  • Product name

  • Description

    Rabbit polyclonal to CAST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human CAST aa 404-454.

  • General notes

    Previously labelled as PAF49.

Properties

Applications

Our Abpromise guarantee covers the use of ab92428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notes
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/100 - 1/1500.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
      Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
    • Sequence similarities

      Belongs to the eukaryotic RPA34 RNA polymerase subunit family.
    • Post-translational
      modifications

      Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
      Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • A34.5 antibody
      • Anti sense to ERCC 1 protein antibody
      • Antisense to ERCC-1 protein antibody
      • ASE 1 antibody
      • ASE-1 antibody
      • ASE1 antibody
      • CAST antibody
      • CD3 epsilon associated protein antibody
      • CD3-epsilon-associated protein antibody
      • CD3E antigen, epsilon polypeptide associated protein antibody
      • CD3E associated protein antibody
      • CD3e molecule, epsilon associated protein antibody
      • CD3E-associated protein antibody
      • CD3EAP antibody
      • DNA directed RNA polymerase I subunit RPA34 antibody
      • DNA-directed RNA polymerase I subunit RPA34 antibody
      • MGC118851 antibody
      • PAF 49 antibody
      • PAF49 antibody
      • RNA polymerase I associated factor PAF49 antibody
      • RNA polymerase I-associated factor PAF49 antibody
      • RPA34_HUMAN antibody
      see all

    Images

    • Formaldehyde fixed asynchronous HeLa cells detected with ab92428 at 1/250 dilution

    References

    ab92428 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92428.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up