Overview

  • Product name

    Cat IgE ELISA Kit
    See all IgE kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum < 10%
    Inter-assay
    Sample n Mean SD CV%
    Serum < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.73 ng/ml
  • Range

    12.5 ng/ml - 400 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 12.5ng/ml - 400ng/ml

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Cat
  • Product overview

    Abcam's IgE Cat ELISA Kit (ab190522) a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgE in Cat plasma and serum.

     

    In this assay the IgE present in samples reacts with the anti-IgE antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    IgE Cat Antibody coated microwells 1 x 96 tests
    IgE Cat Calibrator (Lyophilized) 1 vial
    IgE Cat HRP Conjugate 1 vial
    Stop Solution 1 x 12ml
    Chromogen Substrate Solution 1 x 12ml
  • Research areas

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization

    Secreted
  • Alternative names

    • Ig epsilon chain C region
    • IgE
    • IGHE
    • immunoglobulin heavy constant epsilon

Applications

Our Abpromise guarantee covers the use of ab190522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab190522 (IgE Cat ELISA Kit).

Protocols

References

ab190522 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190522.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up