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|Sample type||Average %||Range|
|Serum||> 85||12.5ng/ml - 800ng/ml|
Abcam's IgG Cat ELISA Kit (ab190523) is an in vitro enzyme linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in biological samples of cats.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo¬genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|IgG Cat Antibody coated microwells||1 x 96 tests|
|IgG Cat Calibrator (lyophilized)||1 vial|
|IgG Cat HRP Conjugate||1 vial|
|Stop Solution||1 x 12ml|
|Chromogen Substrate Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab190523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab190523 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"