Anti-CAT1 antibody (ab37588)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-CAT1 antibody
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DescriptionRabbit polyclonal to CAT1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-FoFr, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CAT1 (gene: SLC7A1).
Read Abcam's proprietary immunogen policy (Peptide available as ab37587.) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: Jurkat, MCF-7 and HepG2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab37588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/1000. | |
| WB | 1/250. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Abcam recommends using milk as the blocking agent. |
Target
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FunctionHigh-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 6541 Human
- Entrez Gene: 11987 Mouse
- Omim: 104615 Human
- SwissProt: P30825 Human
- SwissProt: Q09143 Mouse
- Unigene: 14846 Human
- Unigene: 275489 Mouse
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Alternative names
- Amino acid transporter cationic 1 antibody
- ATRC1 antibody
- CAT-1 antibody
see all
Images
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Western blot - Anti-CAT1 antibody (ab37588)All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml
Lane 1 : Jurkat (Human) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 4 : LNCaP (Human prostate carcinoma) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 160 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab37588 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Western blot - Anti-CAT1 antibody (ab37588)All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Western blot - Anti-CAT1 antibody (ab37588)All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml (3% Milk)
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 135 kDa, 155 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab37588 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CAT1 antibody (ab37588)This image is courtesy of an abreview submitted by Sophie Pezet, ESPCI, FranceIHC-FoFr image of CAT1 staining on Mouse Spinal Cord sections using ab37588 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
References
This product has been referenced in:
- Mitchell CJ et al. Minimal dose of milk protein concentrate to enhance the anabolic signalling response to a single bout of resistance exercise; a randomised controlled trial. J Int Soc Sports Nutr 14:17 (2017). WB ; Human . Read more (PubMed: 28603468) »
- Sheen JM et al. Combined Intraperitoneal and Intrathecal Etanercept Reduce Increased Brain Tumor Necrosis Factor-Alpha and Asymmetric Dimethylarginine Levels and Rescues Spatial Deficits in Young Rats after Bile Duct Ligation. Front Cell Neurosci 10:167 (2016). WB . Read more (PubMed: 27445694) »
