Anti-CAT1 antibody (ab37588)
- Datasheet
- References (9)
- Protocols
Overview
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Product name
Anti-CAT1 antibody -
Description
Rabbit polyclonal to CAT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-FoFr, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide corresponding to Human CAT1 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab37587) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: Jurkat, MCF-7 and HepG2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab37588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/1000. | |
| WB | 1/250. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Abcam recommends using milk as the blocking agent. |
Target
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Function
High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6541 Human
- Entrez Gene: 11987 Mouse
- Omim: 104615 Human
- SwissProt: P30825 Human
- SwissProt: Q09143 Mouse
- Unigene: 14846 Human
- Unigene: 275489 Mouse
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Alternative names
- Amino acid transporter cationic 1 antibody
- ATRC1 antibody
- CAT-1 antibody
see all
Images
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Western blot - Anti-CAT1 antibody (ab37588)All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml
Lane 1 : Jurkat (Human) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 4 : LNCaP (Human prostate carcinoma) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 160 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab37588 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Western blot - Anti-CAT1 antibody (ab37588)All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Western blot - Anti-CAT1 antibody (ab37588)All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/ml (3% Milk)
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 135 kDa, 155 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab37588 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CAT1 antibody (ab37588)This image is courtesy of an abreview submitted by Sophie Pezet, ESPCI, FranceIHC-FoFr image of CAT1 staining on Mouse Spinal Cord sections using ab37588 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
References
This product has been referenced in:
- Augusto L et al. Regulation of arginine transport by GCN2 eIF2 kinase is important for replication of the intracellular parasite Toxoplasma gondii. PLoS Pathog 15:e1007746 (2019). Read more (PubMed: 31194856) »
- Persson P et al. Cellular transport of l-arginine determines renal medullary blood flow in control rats, but not in diabetic rats despite enhanced cellular uptake capacity. Am J Physiol Renal Physiol 312:F278-F283 (2017). Read more (PubMed: 27927650) »
