This antibody produces a beautiful cytoplasmic staining (1:1000 diluted) in the mouse spinal cord. The picture shows the staining obtained at the level of the motorneurons (ventral horn).
The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Dr. Sophie Pezet
Submitted May 17 2011
Get resources and offers direct to your inboxSign up