Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CAT1 antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Specification
Spinal cord

Other product details

Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
This antibody produces a beautiful cytoplasmic staining (1:1000 diluted) in the mouse spinal cord. The picture shows the staining obtained at the level of the motorneurons (ventral horn).

The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

Dr. Sophie Pezet

Verified customer

Submitted May 17 2011

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