Flow Cytometry abreview for Anti-CAT1 antibody

Inconclusive
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (T cell)
Specification
T cell
Permeabilization
No
Gating Strategy
living cells

Other product details

Dilution
1/50
Incubation time
15 minute(s) · Temperature: 4°C · Diluent: PBS+2%FCS

Secondary antibody

Name
Non-Abcam antibody was used: sheep anti rabbit IgG:DyLight649
Host species: Sheep
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/50

Additional data

Additional Notes
The antibody only showed 3% of EcoR+ cells in the mouse Tcell line 2B4 which should contain 100% EcoR.
Abcam response
Thank you for submitting an Abreview for ab37588 in the untested application of Flow cytometry. Based on these results, the antibody may not be suitable for this application.

Ms. Beck

Verified customer

Submitted Sep 09 2011

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