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Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)

Reviews (2)Q&A (29)References (55)
Functional Studies - Catalase Assay Kit (ab83464)
  • Functional Studies - Catalase Assay Kit (ab83464)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric/Fluorometric
  • Platform: Microplate reader
  • Assay time: 40 min
  • Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine

Overview

  • Product name

    Catalase Activity Assay Kit (Colorimetric/Fluorometric)
    See all Catalase kits

  • Detection method

    Colorimetric/Fluorometric

  • Sample type

    Urine, Serum, Plasma, Cell Lysate, Tissue Lysate

  • Assay type

    Enzyme activity (quantitative)

  • Assay time

    0h 40m

  • Species reactivity

    Reacts with: Mammals

  • Product overview

    Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.


    In the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex/Em = 535/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 µU of catalase activity.


    Catalase activity assay protocol summary:
    - add samples and standards to wells
    - add stop solution into sample control wells and incubate at 25ºC for 5 min
    - add H2O2 solution into wells and incubate for 30 min at 25ºC
    - add stop solution
    - add developer mix and incubate for 10 min at 25ºC
    - analyze with microplate reader

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.

  • Platform

    Microplate reader

Properties

Images

  • Functional Studies - Catalase Assay Kit (ab83464)
    Functional Studies - Catalase Assay Kit (ab83464)
    Sample tests using ab83464.
  • Functional Studies - Catalase Assay Kit (ab83464)
    Functional Studies - Catalase Assay Kit (ab83464)
    H2O2 standard curve using ab83464.

References (55)

Publishing research using ab83464? Please let us know so that we can cite the reference in this datasheet.

ab83464 has been referenced in 55 publications.

  • Almatroodi SA  et al. 6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation. Pharmaceutics 13:N/A (2021). PubMed: 33670981
  • Lagnado A  et al. Neutrophils induce paracrine telomere dysfunction and senescence in ROS-dependent manner. EMBO J 40:e106048 (2021). PubMed: 33764576
  • Kim J  et al. Intense Pulsed Light Attenuates UV-Induced Hyperimmune Response and Pigmentation in Human Skin Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33804685
  • Boysen JM  et al. The Peroxiredoxin Asp f3 Acts as Redox Sensor in Aspergillus fumigatus. Genes (Basel) 12:N/A (2021). PubMed: 33946853
  • El-Nobi G  et al. Synbiotic Effects of Saccharomycescerevisiae, Mannan Oligosaccharides, and ß-Glucan on Innate Immunity, Antioxidant Status, and Disease Resistance of Nile Tilapia, Oreochromis niloticus. Antibiotics (Basel) 10:N/A (2021). PubMed: 34065896
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Measurement of the Catalase activity suggests a higher secretion level in a Gram positive mutant

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
The highly sensitive and direct measurement of catalase activity in bacterial supernatants was performed by using the Catalase Activity Assay Kit (ab83464) from Abcam. Bacterial cells were grown in defined medium and harvested at late exponential phase by centrifugation at 15,000 x g for 20 min. The obtained supernatant was sterile filtrated with a 0.22 µm filter and was used for the assay. Standard preparation and assay procedure were done according to manufacturer‘s instruction for colorimetric assays. There were significant differences in the activity of catalase between bacterial mutant 1 and the wildtype. This suggests a higher catalase secretion in mutant 1. Compared to this observation, there are no differences by measuring the Superoxide dismutase activitiy (see see Superoxide dismutase acitivity assay from Abcam [ab65354])
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Lisa Teubner

Verified customer

Submitted Mar 18 2019

Determination of catalase activity in microalgae samples.

 Excellent Good 4/5 (Ease of Use)
Abreviews
abreview image
Sample preparation – Microalgae.
1. Harvest the amount of microalgae necessary for each assay (initial recommendation = 15 mg).
2. Resuspend microalgae in 500 μL of cold Assay Buffer.
3. Sonicate the sample for 30 seconds on ice. Repeat three times.
4. Centrifuge 10 minutes at 4ºC at 10,000g using a cold microcentrifuge to remove any insoluble material.
5. Collect supernatant and transfer to a clean tube.
6. Keep on ice.

- It is recommended to perform several dilution of your sample to ensure the reading are within the standard value range. In this case the dilution factor was 1:100.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Laura Baselga

Verified customer

Submitted Aug 31 2018

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