Product nameAnti-Catalase antibody
See all Catalase primary antibodies
DescriptionRabbit polyclonal to Catalase
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Goat, Dog, Ferret, Macaque monkey, Orangutan
Recombinant human protein purified from E.coli
- HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab16731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/2000. Predicted molecular weight: 60 kDa.|
FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similaritiesBelongs to the catalase family.
modificationsThe N-terminus is blocked.
- Information by UniProt
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
Western Blot analysis of cell lysates.
Lane 1: HeLa cell lysates
Lane 2: Jurkat cell lysates
Lane 3: Mouse brain
Lane 4: Rat brain
The band marked with NS is probably non-specific.
ICC/IF image of ab16731 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab16731 staining human normal adrenal gland tissue. Staining is localised to intracellular compartment (peroxisomes).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification
All lanes : Anti-Catalase antibody (ab16731) at 1/2000 dilution
Lane 1 : 40ug supernatant of mouse liver homogenate
Lane 2 : 20ug supernatant of mouse liver homogenate
Lane 3 : 5ug supernatant of mouse liver homogenate
All lanes : HRP conjugated donkey anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 1 minute
This image is courtesy of an Abreview submitted by Sandra Sobocanec on 16 March 2006.
ab16731 at 1/200 dilution staining Catalase in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used undiluted as secondary.
ab16731 at a 1/200 dilution staining Catalase in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence, incubated for 9 hours at 4°C. Formalin fixed. Blocked with 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green). Nuclei stained with DAPI (blue).
ab16731 staining Catalase in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 before blocking with 2% BSA for 30 minutes at 200C. The sample was incubated with primary antibody (1/200) for 9 hours at 40C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution. DAPI was used to stain the cell nuclei (blue).
This product has been referenced in:
- Krishnan N et al. Harnessing insulin- and leptin-induced oxidation of PTP1B for therapeutic development. Nat Commun 9:283 (2018). Read more (PubMed: 29348454) »
- Kittivorapart J et al. Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/ß-thalassemic patients. Blood Adv 2:95-104 (2018). Read more (PubMed: 29365317) »