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Thanks for looking into this. In answer to your questions, I have also at times loaded 4-5ug/lane. I have also checked it with ponceau stain. I have also cut my membrane and probed for other antibodies and they have worked.
I am more than willing to try a new antibody for Catalse if you suggest it works better or atleast shows bands. I would also like a positive control for catalse just in case.
Thanks and regards,
Asked on Feb 29 2012
Thank you for your reply.
We have a couple of other catalase antibodies that seem to work well, ab1877 or ab16731-
We have tested this antibodies in liver, placenta, or HeLa lysate and any of them will be appropriate as a positive control. Which would you like to try?
I look forward to hearing from you. Please let know if you have any questions or need anything else from me.
Answered on Feb 29 2012