Question (38133) | Anti-Catalase antibody (ab52477)

Go to datasheet (ab52477)

Question

Thanks for looking into this. In answer to your questions, I have also at times loaded 4-5ug/lane. I have also checked it with ponceau stain. I have also cut my membrane and probed for other antibodies and they have worked.
I am more than willing to try a new antibody for Catalse if you suggest it works better or atleast shows bands. I would also like a positive control for catalse just in case.
Thanks and regards,

Answer

Thank you for your reply.

We have a couple of other catalase antibodies that seem to work well, ab1877 or ab16731-

https://www.abcam.com/Catalase-antibody-Peroxisome-Marker-ab1877.html

https://www.abcam.com/Catalase-antibody-ab16731.html

We have tested this antibodies in liver, placenta, or HeLa lysate and any of them will be appropriate as a positive control. Which would you like to try?

I look forward to hearing from you. Please let know if you have any questions or need anything else from me.

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