Western blot abreview for Anti-Catalase antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF F9 GC1 NIH-3T3)
Loading amount
20 µg
Specification
MEF F9 GC1 NIH-3T3
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Other product details

Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA, PBS-Tween 0.1%

Secondary antibody

Name
Non-Abcam antibody was used: Swine anti-Rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Supersignal West Dura
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa

Additional data

Additional Notes
Order: (1) MEF (2) F9 (3) GC1 (4) NIH-3T3
Detects a species of the predicted mobility in a number of mouse cell lines.

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Submitted Aug 02 2010

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