Western blot abreview for Anti-Catalase antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human cancer cell lines)
Loading amount
20 µg
Specification
Human cancer cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Other product details

Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, PBS-Tween 0.1%

Secondary antibody

Name
Non-Abcam antibody was used: Swine anti Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Supersignal west dura
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa

Additional data

Additional Notes
Order: (1) 293T, (2) HeLa, (3) U251, (4) Jurkat, (5) U2OS

Detects a species of the predicted mobility in a range of human cancer lines.

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Submitted Feb 20 2012

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