Western blot abreview for Anti-Catalase antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (Isolated mitochondria)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Specification
Isolated mitochondria
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in TBST
Dilution
1/1500

Secondary antibody

Dilution
1/2000
Name

Detection

Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 60 kDa Non-specific: 50 kDa

Additional data

Additional Notes
Both lanes 1 and 2 represent isolated mitochondria from wild type liver. The dark band is at 60kDa and there is another band around the 50kDa mark.

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Submitted Nov 28 2017

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