Western blot abreview for Anti-Catalase antibody

Excellent
Abreviews
Application
Western blot
Sample
Grey seal Tissue lysate - whole (Right ventricle, brain cortex, kidney)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Right ventricle, brain cortex, kidney
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Licor Odyssey IRDye 800CW Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Licor Odyssey Scanner
Negative control
none
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Positive control
Sheep kidney

Additional data

Additional Notes
Order of samples from left to right: Weddell seal pup: right ventricle, brain cortex, kidney, Weddell seal adult: right ventricle, brain cortex, kidney, Sheep: right ventricle, brain cortex, kidney
Abcam response
Awesome results! - Crystal

Annabelle Batten

Verified customer

Submitted Oct 23 2018

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