Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

Overview

  • Product name

    Anti-Catalase antibody [EP1929Y] - BSA and Azide free
    See all Catalase primary antibodies
  • Description

    Rabbit monoclonal [EP1929Y] to Catalase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human Catalase

  • Positive control

    • WB: HeLa cell lysate. IHC-P: human brain tissue.
  • General notes

    Ab227116 is the carrier-free version of ab76024. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227116 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Properties

Applications

Our Abpromise guarantee covers the use of ab227116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Human Catalase peptide (ab225865).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
    • Involvement in disease

      Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
    • Sequence similarities

      Belongs to the catalase family.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Cellular localization

      Peroxisome.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Images

    • Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76024).

    • This WB data was generated using the same anti-Catalase antibody clone, EP1929Y, in a different buffer formulation (cat# ab76024).

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: CAT knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    References

    This product has been referenced in:

    • Ma HP Hydrogen peroxide stimulates the epithelial sodium channel through a phosphatidylinositide 3-kinase-dependent pathway. J Biol Chem 286:32444-53 (2011). WB ; Xenopus laevis . Read more (PubMed: 21795700) »
    • Kemp K  et al. Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide scavenging enzymes in friedreich ataxia fibroblasts. PLoS One 6:e26098 (2011). WB ; Human . Read more (PubMed: 22016819) »
    See all 2 Publications for this product

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