Recombinant Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 948366. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Thank you for contacting us.

I would like to apologize for this error. When we received the information, that the antibody has been tested in the laboratory with negative results on rat tissue, we updated immediately the datasheet. This was last automn. Indeed, I do not know why we have not sent out the emails to the customers to inform about the issue. I would double check this with the responsable people. Thank you.

I am very sorry that the customerhad problems with this antibody. Please remind the customer, that if in the future he/she has some problems with any of our product, to contact us as soon as possible. Our technical support team is very happy to help to troubleshoot and improve results. In the case an antibody is not working as described, we are happy to replace it (in accordance with our Abpromise guarantee).

Please confirm the order number (or approximate date) of the order of this antibody and I will arrange for the credit. If instead of a credit notethe customerwould prefer an alternative antibody as recompensation, please do let me know.

Thank you for your cooperation and support in this case. I am looking forward to hear back from you onhow you would like to proceed.

The ab16731 is not specific to N-terminal end of the protein. It is produced using recombinant fragment of whole protein and because it is a polyclonal antibody so it will bind to multiple epitopes. It is likely that ab16731 will be able to detect the catalase in your sample. However we can not 100% confirm this without comparison with positive control. I am sending you the lysates ab29545 (Hela cell lysates) which you can use as positive control. I would also recommend trying ab98210 side by side which will help to determine whether the lysates were problematic or antibodies. The Order number I have created for these products is 975321. You will be receiving these products on Monday. I will look forward to receiving the results soon.  

Thank you for sending the image which is very self explanatory for multiple bands. Please ignore my suggestions for multiple bands as these were more general rather than related to this case. The antibody (ab98210) I recommended was the second most suitable antibody based on your immunogen requirements. I am sorry that the antibody did not give expected results. In order to help further I have compiled an excel file of all the anti-catalase antibodies we have in catalogue, along with their immunogen details. Could you please have a look and choose the antibody you would like to try. Unfortunately the immunogen of all these antibodies is from human Catalase protein which is not 100% homologues to Rat protein or at least in the sequence requirement as per your mass spec data. I therefore would be happy to refund the money in case you could not find the product which is a perfect match. Please click to open the sequence similarities info between Rat and Human protein; http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111027-112818-0636-62047582-pg Please choose product from the attached list and contact me for replacement. I would again suggest using human lysates as a positive control. I hope these suggestions will be helpful. Should you have question please do not hesitate to ask.  

Thank you for your email. I am sorry to hear that the results did not improve. The multiple band problem could be due to following reasons: - High protein conc. of lysates per well - High conc. of primary and secondary ab - Cell confluence or cell passage - Cleavage products -  Active protease inhibitors etc.  I would like also to ask about the step you have altered for troubleshooting purpose. Could you send me an image and lysates details? Have you used any positive control lysates also? Looking forward to hearing from you soon.

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. I have been discussing this issue with some colleagues in the Lab. Regrettably, I have been informed that ab76024 is no longer recommended for use in rat samples based on internal test results. I have just amended the on-line datasheet to reflect this. I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful. I am very sorry for this discrepancy and can certainly offer you either an immediate refund or a credit note which you can use in the future. Please do let me know how you wish to proceed with your enquiry.