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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

Reviews (1)Q&A (6)References (14)
Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
  • Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
  • Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
  • Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Catalase antibody [EP1929Y] - Peroxisome Marker
    See all Catalase primary antibodies

  • Description

    Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Catalase aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: P04040
    (Peptide available as ab225865)

  • Positive control

    • WB: HeLa cell lysate. IHC-P: human brain tissue, human bladder cancer tissue. ICC/IF: HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
WB (1)
1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

  • Involvement in disease

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.

  • Sequence similarities

    Belongs to the catalase family.

  • Post-translational
    modifications

    The N-terminus is blocked.

  • Cellular localization

    Peroxisome.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Images

    • Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/10000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : CAT knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 60 kDa



      Lanes 1-2: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab76024 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker was shown to specifically react with Catalase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265250 (knockout cell lysate ab256859) was used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76024 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

      Immunohistochemical analysis of Paraffin-embedded human bladder cancer tissue sections labeling Catalase with ab76024 at 1/1000. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

      Granular cytoplasmic staining on human bladder cancer.

    • Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : CAT knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 60 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Catalase ab76024 at 1/100. Cells were fixed with 100% Methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. 

      ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/100 was used as counterstain antibody.

      Confocal image showing membranous staining in HeLa cells.

       

    • Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

      Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
      Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

    References (14)

    Publishing research using ab76024? Please let us know so that we can cite the reference in this datasheet.

    ab76024 has been referenced in 14 publications.

    • Nørgård MØ  et al. Phenformin Attenuates Renal Injury in Unilateral Ureteral Obstructed Mice without Affecting Immune Cell Infiltration. Pharmaceutics 12:N/A (2020). PubMed: 32224876
    • Xu J  et al. Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion. Oxid Med Cell Longev 2020:6240125 (2020). PubMed: 33299528
    • Wang T  et al. Synergistic antitumour effects of triptolide plus 10-hydroxycamptothecin onbladder cancer. Biomed Pharmacother 115:108899 (2019). PubMed: 31063955
    • Cai M  et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . PubMed: 29458144
    • Kampen KR  et al. The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia N/A:N/A (2018). PubMed: 29930300
    View all Publications for this product

    Customer reviews and Q&As

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    Western blot abreview for Anti-Catalase antibody [EP1929Y] - Peroxisome Marker

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 lung cancer cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-glycine)
    Loading amount
    15 µg
    Specification
    A549 lung cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 21 2015

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