Product nameAnti-Catalase antibody [EP1929Y] - Peroxisome Marker
See all Catalase primary antibodies
DescriptionRabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
Tested applicationsSuitable for: WB, ICC, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
- WB: HeLa cell lysate. IHC-P: human brain tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab76024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Human Catalase peptide (ab225865).|
|ICC||1/100 - 1/250.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similaritiesBelongs to the catalase family.
modificationsThe N-terminus is blocked.
- Information by UniProt
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CAT knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.
This product has been referenced in:
- Cai M et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . Read more (PubMed: 29458144) »
- Kampen KR et al. The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia N/A:N/A (2018). Read more (PubMed: 29930300) »