Validated using a knockout cell line
Recombinant
RabMAb

Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

Knockout Tested Rabbit recombinant monoclonal Catalase antibody [EP1929Y]. Validated in WB, IHC, ICC and tested in Human. Cited in 8 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name
    Anti-Catalase antibody [EP1929Y] - Peroxisome Marker
    See all Catalase primary antibodies
  • Description
    Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Catalase aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: P04040
    (Peptide available as ab225865)

  • Positive control
    • WB: HeLa cell lysate. IHC-P: human brain tissue.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Human Catalase peptide (ab225865).
ICC 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
    • Involvement in disease
      Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
    • Sequence similarities
      Belongs to the catalase family.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localization
      Peroxisome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: CAT knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa

    • Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

    References

    This product has been referenced in:
    • Cai M  et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . Read more (PubMed: 29458144) »
    • Kampen KR  et al. The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia N/A:N/A (2018). Read more (PubMed: 29930300) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    Filter by Application

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    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 lung cancer cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-glycine)
    Loading amount
    15 µg
    Specification
    A549 lung cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 21 2015

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