Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
Knockout Tested Rabbit recombinant monoclonal Catalase antibody [EP1929Y]. Validated in WB, IHC, ICC and tested in Human. Cited in 8 publication(s). Independently reviewed in 1 review(s).
- Datasheet
- References (8)
- Protocols
Overview
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Product nameAnti-Catalase antibody [EP1929Y] - Peroxisome Marker
See all Catalase primary antibodies -
DescriptionRabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Human
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Immunogen
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Positive control
- WB: HeLa cell lysate. IHC-P: human brain tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1929Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab76024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Human Catalase peptide (ab225865). | |
| ICC | 1/100 - 1/250. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
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Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
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Sequence similaritiesBelongs to the catalase family.
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Post-translational
modificationsThe N-terminus is blocked. -
Cellular localizationPeroxisome.
- Information by UniProt
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Database links
- Entrez Gene: 847 Human
- Omim: 115500 Human
- SwissProt: P04040 Human
- Unigene: 502302 Human
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Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Images
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![Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-282492-ab76024GDBKOWB.jpg)
Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CAT knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024_1.JPG)
Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/catalase2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Cai M et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). ICC/IF . Read more (PubMed: 29458144) »
- Kampen KR et al. The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia N/A:N/A (2018). Read more (PubMed: 29930300) »
