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I looked over your antibody list. Thank you for providing that. Based on my mass spec peptide coverage and the rat to human homology it looks like N-term raised antibodies are the best. I found ab16731 to look the most promising there is even a western on the website of rat liver tissue with a very clean one band! I am not sure to which antibody on your excel sheet this matches to but I think it is #27. Anyways if you could send ab16731 ASAP, if you think it is a good one for me to try? There are so many antibodies to try for catalase but this had a lot of different tissue types from rat that it has worked in so hopefully it will work in kidney. Also I have no human lysates to use for a control could you send me some? Thank you for all your help.
Asked on Oct 28 2011
The ab16731 is not specific to N-terminal end of the protein. It is produced using recombinant fragment of whole protein and because it is a polyclonal antibody so it will bind to multiple epitopes. It is likely that ab16731 will be able to detect the catalase in your sample. However we can not 100% confirm this without comparison with positive control. I am sending you the lysates ab29545 (Hela cell lysates) which you can use as positive control. I would also recommend trying ab98210 side by side which will help to determine whether the lysates were problematic or antibodies. The Order number I have created for these products is 975321. You will be receiving these products on Monday. I will look forward to receiving the results soon.
Answered on Oct 28 2011