Anti-Catalase antibody [EPR1928Y] (ab76110)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Catalase antibody [EPR1928Y]
See all Catalase primary antibodies -
DescriptionRabbit monoclonal [EPR1928Y] to Catalase
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Catalase (N terminal). The exact sequence is proprietary.
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Positive control
- TF1, HeLa, Jurkat and HepG2 whole cell lysate (ab7900); human liver tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR1928Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab76110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
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Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
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Sequence similaritiesBelongs to the catalase family.
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Post-translational
modificationsThe N-terminus is blocked. -
Cellular localizationPeroxisome.
- Information by UniProt
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Database links
- Entrez Gene: 847 Human
- Entrez Gene: 12359 Mouse
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: P04040 Human
- SwissProt: P24270 Mouse
- SwissProt: P04762 Rat
- Unigene: 502302 Human
see all -
Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Images
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![Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)](https://www.abcam.com/ps/products/76/ab76110/Images/ab76110-266718-ab76110KOWB.jpg)
Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)](https://www.abcam.com/ps/products/76/ab76110/Images/ab76110ihc.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue. -
![Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)](https://www.abcam.com/ps/products/76/ab76110/Images/ab76110wb.gif)
Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)All lanes : Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution
Lane 1 : TF cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Datasheets and documents
References
This product has been referenced in:
- Gong ZG et al. Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. J Inorg Biochem 192:62-71 (2018). Read more (PubMed: 30599315) »
- Guo Y et al. Trichostatin A attenuates oxidative stress-mediated myocardial injury through the FoxO3a signaling pathway. Int J Mol Med 40:999-1008 (2017). Read more (PubMed: 28849190) »
