Recombinant Anti-Catalase antibody [EPR1928Y] (ab76110)
![Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)](https://www.abcam.com/ps/products/76/ab76110/Images/ab76110-266718-ab76110KOWB.jpg "Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1928Y] to Catalase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Catalase antibody [EPR1928Y]
See all Catalase primary antibodies -
Description
Rabbit monoclonal [EPR1928Y] to Catalase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Catalase (N terminal). The exact sequence is proprietary.
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Positive control
- TF1, HeLa, Jurkat and HepG2 whole cell lysate (ab7900); human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1928Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 847 Human
- Entrez Gene: 12359 Mouse
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: P04040 Human
- SwissProt: P24270 Mouse
- SwissProt: P04762 Rat
- Unigene: 502302 Human
see all -
Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Images
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Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)](https://www.abcam.com/ps/products/76/ab76110/Images/ab76110ihc.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)](https://www.abcam.com/ps/products/76/ab76110/Images/ab76110wb.gif)
Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)All lanes : Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution
Lane 1 : TF cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
![Anti-Catalase antibody [EPR1928Y] (ab76110)](https://www.abcam.com/ps/products/76/ab76110/Images/ab76110-4-benefits-of-recombinant-antibodies.png)
Anti-Catalase antibody [EPR1928Y] (ab76110)
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab76110 has been referenced in 13 publications.
- Li H et al. Exosomes containing miR-451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury. CNS Neurosci Ther 27:564-576 (2021). PubMed: 33533575
- Zheng T et al. Dexmedetomidine suppresses bupivacaine-induced parthanatos in human SH-SY5Y cells via the miR-7-5p/PARP1 axis-mediated ROS. Naunyn Schmiedebergs Arch Pharmacol 394:783-796 (2021). PubMed: 32989562
- Xiang L et al. Curcumin analog A13 alleviates oxidative stress by activating Nrf2/ARE pathway and ameliorates fibrosis in the myocardium of high-fat-diet and streptozotocin-induced diabetic rats. Diabetol Metab Syndr 12:1 (2020). PubMed: 31921358
- You L et al. Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation. Virulence 11:537-553 (2020). PubMed: 32434419
- Alves R et al. Aerobic exercise modulates cardiac NAD(P)H oxidase and the NRF2/KEAP1 pathway in a mouse model of chronic fructose consumption. J Appl Physiol (1985) 128:59-69 (2020). PubMed: 31647720
