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Tags & Cell Markers Subcellular Markers Organelles Peroxisome
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Catalase antibody [EPR1928Y] (ab76110)

  • Datasheet
Submit a review Submit a question References (8)

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Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)
  • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
  • Anti-Catalase antibody [EPR1928Y] (ab76110)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1928Y] to Catalase
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

You may also be interested in

Protein
Product image
Recombinant Catalase protein (Active) (ab219092)
Knockout
Product image
Human CAT (Catalase) knockout HeLa cell line (ab261816)
Assay
Product image
Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)

View more associated products

Overview

  • Product name

    Anti-Catalase antibody [EPR1928Y]
    See all Catalase primary antibodies
  • Description

    Rabbit monoclonal [EPR1928Y] to Catalase
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Catalase (N terminal). The exact sequence is proprietary.

  • Positive control

    • TF1, HeLa, Jurkat and HepG2 whole cell lysate (ab7900); human liver tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1928Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Peroxisome
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Catalase protein (Active) (ab219092)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
    • Involvement in disease

      Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
    • Sequence similarities

      Belongs to the catalase family.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Cellular localization

      Peroxisome.
    • Target information above from: UniProt accession P04040 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 847 Human
      • Entrez Gene: 12359 Mouse
      • Entrez Gene: 24248 Rat
      • Omim: 115500 Human
      • SwissProt: P04040 Human
      • SwissProt: P24270 Mouse
      • SwissProt: P04762 Rat
      • Unigene: 502302 Human
      • Unigene: 4215 Mouse
      • Unigene: 3001 Rat
      see all
    • Alternative names

      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Images

    • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
      Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)

      ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
      Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
      All lanes : Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution

      Lane 1 : TF cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa

    • Anti-Catalase antibody [EPR1928Y] (ab76110)
      Anti-Catalase antibody [EPR1928Y] (ab76110)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab76110? Please let us know so that we can cite the reference in this datasheet.

    ab76110 has been referenced in 8 publications.

    • Xiang L  et al. Curcumin analog A13 alleviates oxidative stress by activating Nrf2/ARE pathway and ameliorates fibrosis in the myocardium of high-fat-diet and streptozotocin-induced diabetic rats. Diabetol Metab Syndr 12:1 (2020). PubMed: 31921358
    • You L  et al. Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation. Virulence 11:537-553 (2020). PubMed: 32434419
    • Lu MC  et al. CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-?B activation. Redox Biol 26:101266 (2019). PubMed: 31279986
    • Jing W  et al. Phosphate Binder, Ferric Citrate, Attenuates Anemia, Renal Dysfunction, Oxidative Stress, Inflammation, and Fibrosis in 5/6 Nephrectomized CKD Rats. J Pharmacol Exp Ther 367:129-137 (2018). PubMed: 30093458
    • Gong ZG  et al. Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. J Inorg Biochem 192:62-71 (2018). PubMed: 30599315
    • Guo Y  et al. Trichostatin A attenuates oxidative stress-mediated myocardial injury through the FoxO3a signaling pathway. Int J Mol Med 40:999-1008 (2017). PubMed: 28849190
    • Jing W  et al. LCZ696 (Sacubitril/valsartan) ameliorates oxidative stress, inflammation, fibrosis and improves renal function beyond angiotensin receptor blockade in CKD. Am J Transl Res 9:5473-5484 (2017). WB . PubMed: 29312499
    • Baqader NO  et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res 13:4398-423 (2014). WB ; Human . PubMed: 25133973

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