Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Catalase antibody [EPR1928Y] (ab76110)

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Overview

  • Product name

    Anti-Catalase antibody [EPR1928Y]
    See all Catalase primary antibodies

  • Description

    Rabbit monoclonal [EPR1928Y] to Catalase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Catalase (N terminal). The exact sequence is proprietary.

  • Positive control

    • TF1, HeLa, Jurkat and HepG2 whole cell lysate (ab7900); human liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab76110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

    • Involvement in disease

      Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.

    • Sequence similarities

      Belongs to the catalase family.

    • Post-translational
      modifications

      The N-terminus is blocked.

    • Cellular localization

      Peroxisome.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Images

    • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
      Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)

      ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
      Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
      All lanes : Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution

      Lane 1 : TF cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa

    References

    This product has been referenced in:

    • Lu MC  et al. CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-?B activation. Redox Biol 26:101266 (2019). Read more (PubMed: 31279986) »
    • Gong ZG  et al. Trehalose prevents cadmium-induced hepatotoxicity by blocking Nrf2 pathway, restoring autophagy and inhibiting apoptosis. J Inorg Biochem 192:62-71 (2018). Read more (PubMed: 30599315) »
    See all 5 Publications for this product

    Publishing research using ab76110? Please let us know so that we can cite the reference in this datasheet.

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