Recombinant Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20469] to Cathelicidin/CLP
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cathelicidin/CLP antibody [EPR20469]
See all Cathelicidin/CLP primary antibodies -
Description
Rabbit monoclonal [EPR20469] to Cathelicidin/CLP -
Host species
Rabbit -
Specificity
Specificity for Antibacterial peptide LL-37 -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human serum and plasma; Human fetal spleen lysate. IHC-P: Human spleen and tonsil tissues. IP: Human spleen lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20469 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/30.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
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Notes |
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IP
1/30. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa). |
Target
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Function
Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. -
Tissue specificity
Expressed in bone marrow and testis and neutrophils. -
Sequence similarities
Belongs to the cathelicidin family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 820 Human
- Omim: 600474 Human
- SwissProt: P49913 Human
- Unigene: 51120 Human
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Alternative names
- 18 kDa cationic antimicrobial protein antibody
- Antibacterial peptide LL-37 antibody
- Antibacterial protein FALL-39 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 10858248].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils in a blood vessel [PMID: 10858248].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lane 2: 3 minutes; Lane 3: 2 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID 10858248).
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Cathelicidin/CLP was immunoprecipitated from 0.35 mg of human spleen lysate with ab207758 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207758 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human spleen lysate, 10 µg (Input).
Lane 2: ab207758 IP in human spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207758 in human spleen lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab207758 has been referenced in 2 publications.
- Duan Z et al. Role of LL-37 in thrombotic complications in patients with COVID-19. Cell Mol Life Sci 79:309 (2022). PubMed: 35596804
- Wang J et al. Cathelicidin Suppresses Colon Cancer Metastasis via a P2RX7-Dependent Mechanism. Mol Ther Oncolytics 12:195-203 (2019). PubMed: 30847383