Recombinant Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free
See all Cathelicidin/CLP primary antibodies -
Description
Rabbit monoclonal [EPR20469] to Cathelicidin/CLP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cathelicidin/CLP aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P49913 -
General notes
ab240378 is the carrier-free version of ab207758 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Cathelicidin
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20469 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa). | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. -
Tissue specificity
Expressed in bone marrow and testis and neutrophils. -
Sequence similarities
Belongs to the cathelicidin family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 820 Human
- Omim: 600474 Human
- SwissProt: P49913 Human
- Unigene: 51120 Human
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Alternative names
- 18 kDa cationic antimicrobial protein antibody
- Antibacterial peptide LL-37 antibody
- Antibacterial protein FALL-39 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)](https://www.abcam.com/ps/products/240/ab240378/Images/ab240378-3-ab207758277647207758IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 10858248].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207758).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)](https://www.abcam.com/ps/products/240/ab240378/Images/ab240378-2-ab207758277646207758IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils in a blood vessel [PMID: 10858248].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207758).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)](https://www.abcam.com/ps/products/240/ab240378/Images/ab240378-1-ab207758277645207758IP.jpg)
Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)Cathelicidin/CLP was immunoprecipitated from 0.35 mg of human spleen lysate with ab207758 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207758 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human spleen lysate, 10 µg (Input).
Lane 2: ab207758 IP in human spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207758 in human spleen lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207758).
Datasheets and documents
References
ab240378 has not yet been referenced specifically in any publications.
