Overview

  • Product name

  • Description

    Rabbit polyclonal to Cathepsin B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cathepsin B aa 198-227 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: P07858

  • Positive control

    • Human liver and hepatocarcinoma tissues; HepG2 cells; Y79 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab190077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 38 kDa.

Target

  • Function

    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similarities

    Belongs to the peptidase C1 family.
  • Cellular localization

    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amyloid precursor protein secretase antibody
    • APP secretase antibody
    • APPS antibody
    • CATB_HUMAN antibody
    • Cathepsin B heavy chain antibody
    • Cathepsin B1 antibody
    • CathepsinB antibody
    • CPSB antibody
    • CTSB antibody
    • cysteine protease antibody
    • OTTHUMP00000116009 antibody
    • OTTHUMP00000229510 antibody
    • OTTHUMP00000229511 antibody
    • OTTHUMP00000229512 antibody
    • OTTHUMP00000229514 antibody
    • OTTHUMP00000229515 antibody
    • OTTHUMP00000229516 antibody
    • Preprocathepsin B antibody
    see all

Images

  • Anti-Cathepsin B antibody (ab190077) at 1/1000 dilution + Y79 cell lysate at 35 µg

    Predicted band size: 38 kDa

  • Flow cytometric analysis of HepG2 cells labeling Cathepsin B with ab190077 at 1/10 dilution (lower histogram) compared to a negative control (upper histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labeling Cathepsin B with ab190077 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Cathepsin B with ab190077 at 10 µg/ml.

References

This product has been referenced in:

  • Li W  et al. Clomiphene citrate induces nuclear translocation of the TFEB transcription factor and triggers apoptosis by enhancing lysosomal membrane permeabilization. Biochem Pharmacol N/A:N/A (2018). Read more (PubMed: 30471247) »
See 1 Publication for this product

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