Key features and details
- Rabbit polyclonal to Cathepsin B
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cathepsin B antibody
See all Cathepsin B primary antibodies
DescriptionRabbit polyclonal to Cathepsin B
Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Cathepsin B aa 198-227 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: P07858
- Human liver and hepatocarcinoma tissues; HepG2 cells; Y79 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 38 kDa.|
FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Sequence similaritiesBelongs to the peptidase C1 family.
Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
Anti-Cathepsin B antibody (ab190077) at 1/1000 dilution + Y79 cell lysate at 35 µg
Predicted band size: 38 kDa
Flow cytometric analysis of HepG2 cells labeling Cathepsin B with ab190077 at 1/10 dilution (lower histogram) compared to a negative control (upper histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labeling Cathepsin B with ab190077 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Cathepsin B with ab190077 at 10 µg/ml.
ab190077 has been referenced in 1 publication.
- Li W et al. Clomiphene citrate induces nuclear translocation of the TFEB transcription factor and triggers apoptosis by enhancing lysosomal membrane permeabilization. Biochem Pharmacol N/A:N/A (2018). PubMed: 30471247