Anti-Cathepsin B antibody (ab30443)
Key features and details
- Rabbit polyclonal to Cathepsin B
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cathepsin B antibody
See all Cathepsin B primary antibodies -
Description
Rabbit polyclonal to Cathepsin B -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified): Cathepsin B from Human liver.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration. PubMed: 24833013
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ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. PubMed: 24833013 |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. |
Target
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Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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Alternative names
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab30443 has been referenced in 5 publications.
- Chien WW et al. Unexpected cross-reactivity of anti-cathepsin B antibodies leads to uncertainties regarding the mechanism of action of anti-CD20 monoclonal antibody GA101. Leuk Res 55:41-48 (2017). PubMed: 28122282
- Han X et al. AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD(+) elevation. Aging Cell 15:416-27 (2016). PubMed: 26890602
- Lohoefer F et al. Quantitative expression and localization of cysteine and aspartic proteases in human abdominal aortic aneurysms. Exp Mol Med 46:e95 (2014). WB, IP ; Human . PubMed: 24833013
- Li C et al. Expression and clinical significance of cathepsin B and stefin A in laryngeal cancer. Oncol Rep 26:869-75 (2011). PubMed: 21667036
- Krohne TU et al. Lipid peroxidation products reduce lysosomal protease activities in human retinal pigment epithelial cells via two different mechanisms of action. Exp Eye Res 90:261-6 (2010). PubMed: 19895809