Western blot abreview for Anti-Cathepsin B antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (283T cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
283T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-tween 0.1% (with 5% milk)
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: AffiniPure Anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 30 and 24 kDa

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Submitted Nov 11 2016

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