• Product name
    Anti-Cathepsin B antibody [CA10]
    See all Cathepsin B primary antibodies
  • Description
    Mouse monoclonal [CA10] to Cathepsin B
  • Host species
  • Specificity
    This antibody recognizes an epitope on the heavy chain of mature Cathepsin B and therefore detects both mature (~31 kDa) and pro (~43 kDa)Cathepsin B.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Pig
  • Immunogen

    Cathepsin B purified from human liver

  • Epitope
    Within the heavy chain of mature cathepsin B
  • Positive control
    • HepG2 cells
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibodies ab214428 or ab125067 as replacements.



Our Abpromise guarantee covers the use of ab58802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa.
IP Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.


  • Function
    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similarities
    Belongs to the peptidase C1 family.
  • Cellular localization
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amyloid precursor protein secretase antibody
    • APP secretase antibody
    • APPS antibody
    • CATB_HUMAN antibody
    • Cathepsin B heavy chain antibody
    • Cathepsin B1 antibody
    • CathepsinB antibody
    • CPSB antibody
    • CTSB antibody
    • cysteine protease antibody
    • OTTHUMP00000116009 antibody
    • OTTHUMP00000229510 antibody
    • OTTHUMP00000229511 antibody
    • OTTHUMP00000229512 antibody
    • OTTHUMP00000229514 antibody
    • OTTHUMP00000229515 antibody
    • OTTHUMP00000229516 antibody
    • Preprocathepsin B antibody
    see all


  • Immunofluorescence analysis of U87MG cells incubated with netrin-1, staining Cathepsin B with ab58802.
  • Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab58802 staining Cathepsin B in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked and then incubated with ab58802 at a 1/200 dilution for 30 minutes at 22°C. The secondary used was a biotin conjugated rabbit anti-mouse monoclonal at a 1/250 dilution.

    See Abreview


This product has been referenced in:
  • Hsu SPC  et al. Temozolomide, sirolimus and chloroquine is a new therapeutic combination that synergizes to disrupt lysosomal function and cholesterol homeostasis in GBM cells. Oncotarget 9:6883-6896 (2018). Read more (PubMed: 29467937) »
  • Komura T  et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . Read more (PubMed: 28898495) »

See all 29 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (gall bladder cancer cell line OCUG-1)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
50 µg
gall bladder cancer cell line OCUG-1
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Mr. Siddharth Mehra

Verified customer

Submitted Jun 01 2016

The immunogen used to create ab58802 is purified cathepsin B from human liver. The epitope has been mapped to the heavy chain of mature cathepsin B, but no more specifics are known.

We have no data showing whether or not this antibody will cro...

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Thank you for your updates.

I am very sorry to hear that the customer is having problem with this antibody as well. It could well be that the product has been damaged during shipment to India. Currently, we have a few alternative antibodies f...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thank you for contacting us.

Unfortunately, alignment of our immunogen with mosquito Cathepsin B shows identity below threshold for reactivity of this antibody in this species. An immunogen should have 85% identity with the target of inter...

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I am very pleased to hear you would like to test ab5076, ab8049, ab18258, ab58802, ab64581, ab82259 and send us your results. The testing discount doesn't apply to ab4674 since this has already been tested in Rhesus monkey and IHC so we would guarantee...

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Thank you for taking time toanswer my questions.

I would like to offer some suggestions to help optimize the results:

1. First thing first: ab77355 is a goat antibody. The secondary antibody will cross reacts with Bovine Ig present in...

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Thank you for contacting us.

Your credit note ID is ******.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in o...

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Thank you for taking time to check these additional details. The details provided will enable us to investigate this case. Itam very sorrythatthe results with this antibodyhave not been successful.

I have checked the information provided and...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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