Overview

  • Product name
    Anti-Cathepsin B antibody [CA10]
    See all Cathepsin B primary antibodies
  • Description
    Mouse monoclonal [CA10] to Cathepsin B
  • Host species
    Mouse
  • Specificity
    This antibody recognizes an epitope on the heavy chain of mature Cathepsin B and therefore detects both mature (~31 kDa) and pro (~43 kDa)Cathepsin B.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Pig
  • Immunogen

    Cathepsin B purified from human liver

  • Epitope
    Within the heavy chain of mature cathepsin B
  • Positive control
    • HepG2 cells
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibodies ab214428 or ab125067 as replacements.

Properties

Applications

Our Abpromise guarantee covers the use of ab58802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa.
IP Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similarities
    Belongs to the peptidase C1 family.
  • Cellular localization
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amyloid precursor protein secretase antibody
    • APP secretase antibody
    • APPS antibody
    • CATB_HUMAN antibody
    • Cathepsin B heavy chain antibody
    • Cathepsin B1 antibody
    • CathepsinB antibody
    • CPSB antibody
    • CTSB antibody
    • cysteine protease antibody
    • OTTHUMP00000116009 antibody
    • OTTHUMP00000229510 antibody
    • OTTHUMP00000229511 antibody
    • OTTHUMP00000229512 antibody
    • OTTHUMP00000229514 antibody
    • OTTHUMP00000229515 antibody
    • OTTHUMP00000229516 antibody
    • Preprocathepsin B antibody
    see all

Images

  • Immunofluorescence analysis of U87MG cells incubated with netrin-1, staining Cathepsin B with ab58802.
  • Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab58802 staining Cathepsin B in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked and then incubated with ab58802 at a 1/200 dilution for 30 minutes at 22°C. The secondary used was a biotin conjugated rabbit anti-mouse monoclonal at a 1/250 dilution.

    See Abreview

References

This product has been referenced in:
  • Hsu SPC  et al. Temozolomide, sirolimus and chloroquine is a new therapeutic combination that synergizes to disrupt lysosomal function and cholesterol homeostasis in GBM cells. Oncotarget 9:6883-6896 (2018). Read more (PubMed: 29467937) »
  • Komura T  et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . Read more (PubMed: 28898495) »

See all 30 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (gall bladder cancer cell line OCUG-1)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
50 µg
Specification
gall bladder cancer cell line OCUG-1
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mr. Siddharth Mehra

Verified customer

Submitted Jun 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Specification
Mammary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Vector M.O.M. Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.2% · Temperature: 22°C
Username

Dr. Irina Teplova

Verified customer

Submitted Jul 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y cell)
Loading amount
50 µg
Specification
SHSY5Y cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A375 cells)
Loading amount
50 µg
Specification
A375 cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 31 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
10 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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