Overview

  • Product name
    Anti-Cathepsin B antibody [CA10]
    See all Cathepsin B primary antibodies
  • Description
    Mouse monoclonal [CA10] to Cathepsin B
  • Host species
    Mouse
  • Specificity
    This antibody recognizes an epitope on the heavy chain of mature Cathepsin B and therefore detects both mature (~31 kDa) and pro (~43 kDa)Cathepsin B.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Pig
  • Immunogen

    Cathepsin B purified from human liver

  • Epitope
    Within the heavy chain of mature cathepsin B
  • Positive control
    • HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab58802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa.
IP Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similarities
    Belongs to the peptidase C1 family.
  • Cellular localization
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amyloid precursor protein secretase antibody
    • APP secretase antibody
    • APPS antibody
    • CATB_HUMAN antibody
    • Cathepsin B heavy chain antibody
    • Cathepsin B1 antibody
    • CathepsinB antibody
    • CPSB antibody
    • CTSB antibody
    • cysteine protease antibody
    • OTTHUMP00000116009 antibody
    • OTTHUMP00000229510 antibody
    • OTTHUMP00000229511 antibody
    • OTTHUMP00000229512 antibody
    • OTTHUMP00000229514 antibody
    • OTTHUMP00000229515 antibody
    • OTTHUMP00000229516 antibody
    • Preprocathepsin B antibody
    see all

Images

  • ab58802 staining Cathepsin B in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked and then incubated with ab58802 at a 1/200 dilution for 30 minutes at 22°C. The secondary used was a biotin conjugated rabbit anti-mouse monoclonal at a 1/250 dilution.

    See Abreview

  • Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/ml + Native human Cathepsin B protein (ab90387) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa


    Exposure time: 10 seconds
  • Immunofluorescence analysis of U87MG cells incubated with netrin-1, staining Cathepsin B with ab58802.
  • Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Li G & Moellering RE A Concise, Modular Antibody-Oligonucleotide Conjugation Strategy Based on Disuccinimidyl Ester Activation Chemistry. Chembiochem N/A:N/A (2019). Read more (PubMed: 30767357) »
  • Chen CH  et al. Study of Cathepsin B inhibition in VEGFR TKI treated human renal cell carcinoma xenografts. Oncogenesis 8:15 (2019). Read more (PubMed: 30796200) »
See all 35 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (gall bladder cancer cell line OCUG-1)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
50 µg
Specification
gall bladder cancer cell line OCUG-1
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Mr. Siddharth Mehra

Verified customer

Submitted Jun 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Specification
Mammary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Vector M.O.M. Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.2% · Temperature: 22°C

Dr. Irina Teplova

Verified customer

Submitted Jul 18 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y cell)
Loading amount
50 µg
Specification
SHSY5Y cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 17 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (A375 cells)
Loading amount
50 µg
Specification
A375 cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 31 2009

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
10 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 27 2009

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