Overview

  • Product name
    Anti-Cathepsin B antibody [CB131]
    See all Cathepsin B primary antibodies
  • Description
    Mouse monoclonal [CB131] to Cathepsin B
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
    Unsuitable for: IHC-Fr or WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • IHC: skin

Properties

Applications

Our Abpromise guarantee covers the use of ab26395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/40 - 1/80. This product does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining.

    Is unsuitable for IHC-Fr or WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • Sequence similarities
      Belongs to the peptidase C1 family.
    • Cellular localization
      Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amyloid precursor protein secretase antibody
      • APP secretase antibody
      • APPS antibody
      • CATB_HUMAN antibody
      • Cathepsin B heavy chain antibody
      • Cathepsin B1 antibody
      • CathepsinB antibody
      • CPSB antibody
      • CTSB antibody
      • cysteine protease antibody
      • OTTHUMP00000116009 antibody
      • OTTHUMP00000229510 antibody
      • OTTHUMP00000229511 antibody
      • OTTHUMP00000229512 antibody
      • OTTHUMP00000229514 antibody
      • OTTHUMP00000229515 antibody
      • OTTHUMP00000229516 antibody
      • Preprocathepsin B antibody
      see all

    References

    This product has been referenced in:
    • Tizon B  et al. Cystatin C protects neuronal cells from amyloid-beta-induced toxicity. J Alzheimers Dis 19:885-94 (2010). Read more (PubMed: 20157244) »
    • van Dekken H  et al. Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet 189:37-42 (2009). Read more (PubMed: 19167610) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for forwarding the complaint form for the cathepsin B antibody. I am sorry to see that the customer has spent so much time trying to get the antibody to work.

    I think the protocol the customer used is generally correct but I am concerned about the blocking step that is specified in the complaint form: methanol for 10 minutes. I have not seen this before, for paraffin-embedded tissue. It is possible that the epitope this antibody recognizes is sensitive to methanol, even after fixation with PFA. Another possibility is that the antigen retrieval steps actually lead to a weaker stain compared to a protocol that uses no antigen retrieval, but I think this is less likely.

    Can you please ask your customer if they have always used methanol for blocking? It is usually used as a fixative for sections of frozen tissue. The antibody has been found to be ineffective for staining frozen tissue, and I suspect the test was on methanol-fixed tissue sections.

    However, I am willing to issue a replacement. A different antibody will probably be best, for instance ab58802:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=58802).

    Read More

    Question
    Answer

    Thank you for your enquiry. Information from our source indicates the immunogen for ab26395 Cathepsin B antibody [CB131] corresponds to the mature form of the peptide. Based on this, I believe the antibody should be able to detect both the pro and mature forms. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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