Recombinant
RabMAb

Anti-Cathepsin B antibody [EPR4323] (ab125067)

Submit an Abreview Q&A (1)Specific References (1)

Overview

  • Product name
    Anti-Cathepsin B antibody [EPR4323]
    See all Cathepsin B primary antibodies
  • Description
    Rabbit monoclonal [EPR4323] to Cathepsin B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • Positive control
    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function
      Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • Sequence similarities
      Belongs to the peptidase C1 family.
    • Cellular localization
      Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
      • Alternative names
        • Amyloid precursor protein secretase antibody
        • APP secretase antibody
        • APPS antibody
        • CATB_HUMAN antibody
        • Cathepsin B heavy chain antibody
        • Cathepsin B1 antibody
        • CathepsinB antibody
        • CPSB antibody
        • CTSB antibody
        • cysteine protease antibody
        • OTTHUMP00000116009 antibody
        • OTTHUMP00000229510 antibody
        • OTTHUMP00000229511 antibody
        • OTTHUMP00000229512 antibody
        • OTTHUMP00000229514 antibody
        • OTTHUMP00000229515 antibody
        • OTTHUMP00000229516 antibody
        • Preprocathepsin B antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.
      • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.
      • Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

        Lane 1 : HT1080 cell lysate
        Lane 2 : A375 cell lysate
        Lane 3 : SW480 cell lysate
        Lane 4 : A549 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 38 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

      References

      This product has been referenced in:
      • Feng X  et al. Genome-wide differential expression profiling of mRNAs and lncRNAs associated with prolificacy in Hu sheep. Biosci Rep 38:N/A (2018). Read more (PubMed: 29439142) »

      See 1 Publication for this product

      Publishing research using ab125067? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      1 Review or Q&A

      10
      Votes: Highest First

      Dear Sirs, you show a picture of HeLa cells incubated with Anti-Cathepsin B
      antibody [EPR4323] (ab125067) and developed in immunofluorescence. May you
      suggest me the method and the secondary antibody used for this staining?
      Thank you in ...

      Read More

      Thank you for contacting us and your interest in our products.
      Please find the ICC protocol used with the Anti-Cathepsin B antibody [EPR4323] (ab125067) attached to this email.
      The secondary antibody used in the staining was an Alexa Fluor 56...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up