Recombinant Anti-Cathepsin B antibody [EPR4323] (ab125067)
![Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139211-anti-cathepsin-b-antibody-epr4323-western-blot.jpg "Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4323] to Cathepsin B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Cathepsin B antibody [EPR4323]
See all Cathepsin B primary antibodies -
Description
Rabbit monoclonal [EPR4323] to Cathepsin B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Cathepsin B aa 1-200. The exact sequence is proprietary.
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Positive control
- Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4323 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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Alternative names
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
Images
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Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution
Lane 1 : HT1080 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139210-anti-cathepsin-b-antibody-epr4323-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139208-anti-cathepsin-b-antibody-epr4323-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139209-anti-cathepsin-b-antibody-epr4323-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-5-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin B antibody [EPR4323] (ab125067)
Protocols
Datasheets and documents
References (11)
ab125067 has been referenced in 11 publications.
- Yang SY et al. Glucocerebrosidase activity, cathepsin D and monomeric a-synuclein interactions in a stem cell derived neuronal model of a PD associated GBA1 mutation. Neurobiol Dis 134:104620 (2020). PubMed: 31634558
- Shen Y et al. Iron Promotes Dihydroartemisinin Cytotoxicity via ROS Production and Blockade of Autophagic Flux via Lysosomal Damage in Osteosarcoma. Front Pharmacol 11:444 (2020). PubMed: 32431605
- Ha Y et al. Induction of Lysosome-associated Protein Transmembrane 4 Beta via Sulfatase 2 Enhances Autophagic Flux in Liver Cancer Cells. Hepatol Commun 3:1520-1543 (2019). PubMed: 31701075
- Rigo A et al. BID and the a-bisabolol-triggered cell death program: converging on mitochondria and lysosomes. Cell Death Dis 10:889 (2019). PubMed: 31767857
- Chen CH et al. Study of Cathepsin B inhibition in VEGFR TKI treated human renal cell carcinoma xenografts. Oncogenesis 8:15 (2019). PubMed: 30796200
