Recombinant Anti-Cathepsin B antibody [EPR4323] (ab125067)
![Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139211-anti-cathepsin-b-antibody-epr4323-western-blot.jpg "Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4323] to Cathepsin B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Cathepsin B antibody [EPR4323]
See all Cathepsin B primary antibodies -
Description
Rabbit monoclonal [EPR4323] to Cathepsin B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Cathepsin B aa 1-200. The exact sequence is proprietary.
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Positive control
- Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4323 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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Alternative names
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
Images
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Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution
Lane 1 : HT1080 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139210-anti-cathepsin-b-antibody-epr4323-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139208-anti-cathepsin-b-antibody-epr4323-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-139209-anti-cathepsin-b-antibody-epr4323-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Cathepsin B antibody [EPR4323] (ab125067)](https://www.abcam.com/ps/products/125/ab125067/Images/ab125067-5-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin B antibody [EPR4323] (ab125067)
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab125067 has been referenced in 12 publications.
- Yang SY et al. Glucocerebrosidase activity, cathepsin D and monomeric a-synuclein interactions in a stem cell derived neuronal model of a PD associated GBA1 mutation. Neurobiol Dis 134:104620 (2020). PubMed: 31634558
- Shen Y et al. Iron Promotes Dihydroartemisinin Cytotoxicity via ROS Production and Blockade of Autophagic Flux via Lysosomal Damage in Osteosarcoma. Front Pharmacol 11:444 (2020). PubMed: 32431605
- Ahsan A et al. Tomatidine protects against ischemic neuronal injury by improving lysosomal function. Eur J Pharmacol 882:173280 (2020). PubMed: 32580039
- Ha Y et al. Induction of Lysosome-associated Protein Transmembrane 4 Beta via Sulfatase 2 Enhances Autophagic Flux in Liver Cancer Cells. Hepatol Commun 3:1520-1543 (2019). PubMed: 31701075
- Rigo A et al. BID and the a-bisabolol-triggered cell death program: converging on mitochondria and lysosomes. Cell Death Dis 10:889 (2019). PubMed: 31767857
