Recombinant
RabMAb

Recombinant Anti-Cathepsin B antibody [EPR4323] (ab125067)

Submit a review Q&A (1)References (7)

Overview

  • Product name

    Anti-Cathepsin B antibody [EPR4323]
    See all Cathepsin B primary antibodies

  • Description

    Rabbit monoclonal [EPR4323] to Cathepsin B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • Positive control

    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab125067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

    • Sequence similarities

      Belongs to the peptidase C1 family.

    • Cellular localization

      Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      • Alternative names

        • Amyloid precursor protein secretase antibody
        • APP secretase antibody
        • APPS antibody
        • CATB_HUMAN antibody
        • Cathepsin B heavy chain antibody
        • Cathepsin B1 antibody
        • CathepsinB antibody
        • CPSB antibody
        • CTSB antibody
        • cysteine protease antibody
        • OTTHUMP00000116009 antibody
        • OTTHUMP00000229510 antibody
        • OTTHUMP00000229511 antibody
        • OTTHUMP00000229512 antibody
        • OTTHUMP00000229514 antibody
        • OTTHUMP00000229515 antibody
        • OTTHUMP00000229516 antibody
        • Preprocathepsin B antibody
        see all

      Images

      • Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

        Lane 1 : HT1080 cell lysate
        Lane 2 : A375 cell lysate
        Lane 3 : SW480 cell lysate
        Lane 4 : A549 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 38 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)

        ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)

        ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Chen CH  et al. Study of Cathepsin B inhibition in VEGFR TKI treated human renal cell carcinoma xenografts. Oncogenesis 8:15 (2019). Read more (PubMed: 30796200) »
      • Feng X  et al. Genome-wide differential expression profiling of mRNAs and lncRNAs associated with prolificacy in Hu sheep. Biosci Rep 38:N/A (2018). Read more (PubMed: 29439142) »
      See all 7 Publications for this product

      Publishing research using ab125067? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Question

      Dear Sirs, you show a picture of HeLa cells incubated with Anti-Cathepsin B
      antibody [EPR4323] (ab125067) and developed in immunofluorescence. May you
      suggest me the method and the secondary antibody used for this staining?
      Thank you in advance.
      --

      Read More
      Answer

      Thank you for contacting us and your interest in our products.
      Please find the ICC protocol used with the Anti-Cathepsin B antibody [EPR4323] (ab125067) attached to this email.
      The secondary antibody used in the staining was an Alexa Fluor 568 goat anti-rabbit IgG. We have several antibodies in our catalogue which may be of use to you as secondary antibodies to ab125067. For example:
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed (ab96896)
      Donkey anti-Rabbit IgG H&L (DyLight® 594) secondary antibody (ab96921)
      Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody (ab98488)
      I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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