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    cathepsin-b-antibody-epr4323-ab125067.pdf

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RecombinantRabMAb

Recombinant Anti-Cathepsin B antibody [EPR4323] (ab125067)

  • Datasheet
Submit a review Q&A (1)References (15)

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Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
  • Anti-Cathepsin B antibody [EPR4323] (ab125067)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4323] to Cathepsin B
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Cathepsin B antibody [EPR4323]
    See all Cathepsin B primary antibodies
  • Description

    Rabbit monoclonal [EPR4323] to Cathepsin B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • Positive control

    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4323
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • ECM enzymes
    • Cathespins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Adhesion molecules ELISA kits
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)
  • Assay kits

    • Cathepsin B Activity Assay Kit (Fluorometric) (ab65300)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • SW480 whole cell lysate (ab3957)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human Cathepsin B protein (ab198475)
  • Related Products

    • Cathepsin B Inhibitor Screening Kit (Fluorometric) (ab185438)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similarities

    Belongs to the peptidase C1 family.
  • Cellular localization

    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1508 Human
    • Omim: 116810 Human
    • SwissProt: P07858 Human
    • Unigene: 520898 Human
    • Alternative names

      • Amyloid precursor protein secretase antibody
      • APP secretase antibody
      • APPS antibody
      • CATB_HUMAN antibody
      • Cathepsin B heavy chain antibody
      • Cathepsin B1 antibody
      • CathepsinB antibody
      • CPSB antibody
      • CTSB antibody
      • cysteine protease antibody
      • OTTHUMP00000116009 antibody
      • OTTHUMP00000229510 antibody
      • OTTHUMP00000229511 antibody
      • OTTHUMP00000229512 antibody
      • OTTHUMP00000229514 antibody
      • OTTHUMP00000229515 antibody
      • OTTHUMP00000229516 antibody
      • Preprocathepsin B antibody
      see all

    Images

    • Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)
      Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067)
      All lanes : Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

      Lane 1 : HT1080 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)

      ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)
      Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (ab125067)
      ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (ab125067)

      ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Cathepsin B antibody [EPR4323] (ab125067)
      Anti-Cathepsin B antibody [EPR4323] (ab125067)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (15)

    Publishing research using ab125067? Please let us know so that we can cite the reference in this datasheet.

    ab125067 has been referenced in 15 publications.

    • Castroflorio E  et al. The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. Cell Mol Life Sci 78:3503-3524 (2021). PubMed: 33340069
    • Li L  et al. Sunitinib treatment promotes metastasis of drug-resistant renal cell carcinoma via TFE3 signaling pathway. Cell Death Dis 12:220 (2021). PubMed: 33637706
    • Shen Y  et al. Iron Promotes Dihydroartemisinin Cytotoxicity via ROS Production and Blockade of Autophagic Flux via Lysosomal Damage in Osteosarcoma. Front Pharmacol 11:444 (2020). PubMed: 32431605
    • Ahsan A  et al. Tomatidine protects against ischemic neuronal injury by improving lysosomal function. Eur J Pharmacol 882:173280 (2020). PubMed: 32580039
    • Zhao Z  et al. LncRNA DLX6-AS1 Promotes Malignant Phenotype and Lymph Node Metastasis in Prostate Cancer by Inducing LARGE Methylation. Front Oncol 10:1172 (2020). PubMed: 32850336
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Dear Sirs, you show a picture of HeLa cells incubated with Anti-Cathepsin B
    antibody [EPR4323] (ab125067) and developed in immunofluorescence. May you
    suggest me the method and the secondary antibody used for this staining?
    Thank you in advance.
    --

    Read More

    Abcam community

    Verified customer

    Asked on Aug 23 2012

    Answer

    Thank you for contacting us and your interest in our products.
    Please find the ICC protocol used with the Anti-Cathepsin B antibody [EPR4323] (ab125067) attached to this email.
    The secondary antibody used in the staining was an Alexa Fluor 568 goat anti-rabbit IgG. We have several antibodies in our catalogue which may be of use to you as secondary antibodies to ab125067. For example:
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed (ab96896)
    Donkey anti-Rabbit IgG H&L (DyLight® 594) secondary antibody (ab96921)
    Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody (ab98488)
    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Aug 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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