Recombinant
RabMAb

Recombinant Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free (ab230401)

Overview

  • Product name

    Anti-Cathepsin B antibody [EPR4323] - BSA and Azide free
    See all Cathepsin B primary antibodies
  • Description

    Rabbit monoclonal [EPR4323] to Cathepsin B - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cathepsin B aa 1-200. The exact sequence is proprietary.

  • Positive control

    • Human kidney and papillary carcinoma tissues; HeLa cells; HT1080, A375, SW480 and A549 cell line lysates.
  • General notes

    Ab230401 is the carrier-free version of ab125067. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230401 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab230401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
    • Sequence similarities

      Belongs to the peptidase C1 family.
    • Cellular localization

      Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • Amyloid precursor protein secretase antibody
      • APP secretase antibody
      • APPS antibody
      • CATB_HUMAN antibody
      • Cathepsin B heavy chain antibody
      • Cathepsin B1 antibody
      • CathepsinB antibody
      • CPSB antibody
      • CTSB antibody
      • cysteine protease antibody
      • OTTHUMP00000116009 antibody
      • OTTHUMP00000229510 antibody
      • OTTHUMP00000229511 antibody
      • OTTHUMP00000229512 antibody
      • OTTHUMP00000229514 antibody
      • OTTHUMP00000229515 antibody
      • OTTHUMP00000229516 antibody
      • Preprocathepsin B antibody
      see all

    Images

    • ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125067).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125067).

    • This IHC data was generated using the same anti-Cathepsin B antibody clone, EPR4323, in a different buffer formulation (cat# ab125067).

      ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • This ICC data was generated using the same anti-Cathepsin B antibody clone, EPR4323, in a different buffer formulation (cat# ab125067).

      ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.

    References

    This product has been referenced in:

    • Wu JS  et al. Cathepsin B defines leader cells during the collective invasion of salivary adenoid cystic carcinoma. Int J Oncol 54:1233-1244 (2019). Read more (PubMed: 30968153) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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