Cathepsin B Inhibitor Screening Kit (Fluorometric) (ab185438)

Overview

  • Product name
    Cathepsin B Inhibitor Screening Kit (Fluorometric)
    See all Cathepsin B kits
  • Detection method
    Fluorescent
  • Sample type
    Inhibitor compounds
  • Assay type
    Semi-quantitative
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Abcam's Cathepsin B Inhibitor Screening Kit (ab185438) utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin B-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This high-throughput adaptable assay kit is simple, sensitive, and rapid tool to screen the potential inhibitors of Cathepsin B.

     

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  • Notes

    Cathepsin B (CTSB, EC 3.4.22.1) is a lysosomal cysteine proteinase that is suggested to participate in intracellular degradation and turnover of proteins. It has also been implicated in tumor invasion and metastasis.

  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Cathepsin B 1 x 5µl
    CTS B Inhibitor (F-F-FMK 1 mM) 1 x 20µl
    CTS B Reagent 1 x 100µl
    CTS B Substrate (Ac-RR-AFC 10 mM) 1 x 0.2ml
    CTSB Reaction Buffer 1 x 15ml
  • Research areas
  • Relevance
    Cathepsin B is a normal lysosomal proteinase that is expressed in all cells. It is a cysteine endoproteinase structurally and functionally related to the papain family of proteases. Cathepsin B is synthesized as an inactive pro enzyme which, by removal of a 62 amino acid propeptide, is activated to the single chain form of 31 kDa or the two chain form (25 and 5 kDa subunits). Matured Cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins at acidic and neutral pH. Endogenous inhibitors of cysteine proteases, the cystatins and stefins, may play a major role in regulating Cathepsin B activity. In tumor cells and in cells exposed to mitogens, cathepsin B displays altered cellular trafficking resulting in the secretion of the 43 kDa precursor. Elevated levels of Cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers. Cathepsin B has also been implicated in the pathogenesis of rhematoid arthritis, muscular dystrophy and tumour metastasis. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP).
  • Cellular localization
    Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Alternative names
    • Amyloid precursor protein secretase
    • APP secretase
    • APPS
    • Cathepsin B1
    • CathepsinB
    • CPSB
    • CTSB
    • Preprocathepsin B
    see all

Associated products

Images

  • Inhibition of Cathepsin B activity by the CTSB control

  • Inhibition of Cathepsin B activity by the Cathepsin B inhibitor E-64

  • Inhibition of Cathepsin B activity by the Cathepsin B inhibitor Chymostatin

  • Inhibition of Cathepsin B activity by the Cathepsin B inhibitor Antipain dihydrochloride

  • Inhibition of Cathepsin B activity by Cathepsin B Inhibitor Screening Kit was performed following kit protocol. This is example data only.

Protocols

References

ab185438 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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