Overview

  • Product name

  • Description

    Rabbit polyclonal to Cathepsin D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Cathepsin D aa 115-141 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    IACWIHHKYNSDKSSTYVKNGTSFDIH


    Database link: P07339

  • Positive control

    • Rat kidney and Human colon carcinoma lysates. U138 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab217310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 45 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/200.

Target

  • Function

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificity

    Expressed in the aorta extrcellular space (at protein level).
  • Involvement in disease

    Ceroid lipofuscinosis, neuronal, 10
  • Sequence similarities

    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • Database links

  • Alternative names

    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Skin with ab217310 at 1/200
  • Immunofluorescence analysis of formalin-fixed U138 cells, labeling Cathepsin D using ab217310 at a 1/50 dilution; followed by goat anti-rabbit lgG (H+L) Alexa Fluor 488-conjugated secondary antibody.

  • All lanes : Anti-Cathepsin D antibody (ab217310) at 1/200 dilution

    Lane 1 : rat kidney lysates
    Lane 2 : Human colon carcinoma lysates

    Secondary
    All lanes : goat anti-rabbit antibody (H+L) HRP-conjugated at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?

References

ab217310 has not yet been referenced specifically in any publications.

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