Key features and details
- Rabbit polyclonal to Cathepsin D
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Cathepsin D antibody
See all Cathepsin D primary antibodies
DescriptionRabbit polyclonal to Cathepsin D
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat kidney and Human colon carcinoma lysates. U138 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 45 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Tissue specificityExpressed in the aorta extrcellular space (at protein level).
Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
Sequence similaritiesBelongs to the peptidase A1 family.
Contains 1 peptidase A1 domain.
modificationsN- and O-glycosylated.
Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
- Information by UniProt
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Skin with ab217310 at 1/200
Immunofluorescence analysis of formalin-fixed U138 cells, labeling Cathepsin D using ab217310 at a 1/50 dilution; followed by goat anti-rabbit lgG (H+L) Alexa Fluor 488-conjugated secondary antibody.
All lanes : Anti-Cathepsin D antibody (ab217310) at 1/200 dilution
Lane 1 : rat kidney lysates
Lane 2 : Human colon carcinoma lysates
All lanes : goat anti-rabbit antibody (H+L) HRP-conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
ab217310 has been referenced in 1 publication.
- Wen M et al. Proteomic Analysis of Rat Cerebral Cortex in the Subacute to Long-Term Phases of Focal Cerebral Ischemia-Reperfusion Injury. J Proteome Res 18:3099-3118 (2019). PubMed: 31265301