Application
Western blot
Sample
Cat Cell lysate - whole cell (CAT-MT and K12- Feline mammary carcinoma, SCCF1)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
CAT-MT and K12- Feline mammary carcinoma, SCCF1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% Milk
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: chicken polyclonal
Host species: Chicken
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Chicken
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 29 kDa Non-specific: 45 kDa
Positive control
MCF7, whole cell lysate (10ug)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Holly Pondenis
Verified customer
Submitted Aug 26 2015