• Product name
  • Description
    Rabbit polyclonal to Cathepsin D
  • Host species
  • Specificity
    This antibody reacts with the zymogen as well as the activated form of cathepsin D. It stains fibroblasts and macrophages strongly but stains weakly with the myoepithelial cells of breast. It also stains sweat ducts and glands in the skin, islets cells and granules of pancreatic acini.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Cathepsin D isolated from human liver

  • Positive control
    • ICC/IF: A431 cells.



Our Abpromise guarantee covers the use of ab826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75 - 1/100. ABC method.
IHC-Fr 1/75 - 1/100. ABC method.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19505575
ICC/IF Use a concentration of 10 µg/ml.


  • Function
    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificity
    Expressed in the aorta extrcellular space (at protein level).
  • Involvement in disease
    Ceroid lipofuscinosis, neuronal, 10
  • Sequence similarities
    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    N- and O-glycosylated.
  • Cellular localization
    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt
  • Database links
  • Alternative names
    • CatD antibody
    • CATD_HUMAN antibody
    • Cathepsin D antibody
    • Cathepsin D heavy chain antibody
    • CD antibody
    • Ceroid lipofuscinosis neuronal 10 antibody
    • CLN10 antibody
    • CPSD antibody
    • ctsd antibody
    • Epididymis secretory sperm binding protein Li 130P antibody
    • HEL S 130P antibody
    • Lysosomal aspartyl peptidase antibody
    • Lysosomal aspartyl protease antibody
    • MGC2311 antibody
    see all


  • ICC/IF image of ab826 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab826, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab826 staining Cathepsin D in formalin-fixed, paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • Immunohistochemical analysis of PFA-perfusion fixed, frozen rat brain tissue sections (substantia nigra), staining Cathepsin D with ab826. Samples were incubated with primary antibody overnight at room temperature.


This product has been referenced in:
  • Minchew CL & Didenko VV Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D. Methods Mol Biol 1554:229-236 (2017). Read more (PubMed: 28185196) »
  • Takakura K  et al. Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions. Heliyon 3:e00462 (2017). WB ; Rat . Read more (PubMed: 29264419) »

See all 4 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Rat Cell lysate - whole cell (brain)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C

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Submitted Oct 27 2014

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Vielen Dank für dieses Update.

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Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

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