Product nameAnti-Cathepsin D antibody [CTD-19]
See all Cathepsin D primary antibodies
DescriptionMouse monoclonal [CTD-19] to Cathepsin D
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Full length native protein (purified) (Human liver).
- Human breast carcinoma tissue or cell line extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.097% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab6313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 52 kDa.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. PubMed: 24833013|
FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Tissue specificityExpressed in the aorta extrcellular space (at protein level).
Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
Sequence similaritiesBelongs to the peptidase A1 family.
Contains 1 peptidase A1 domain.
modificationsN- and O-glycosylated.
Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
- Information by UniProt
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg
HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28,48,50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
ab6313 staining Cathepsin D in DU145 human prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 and blocked with 5% BSA at room temperature for 20 minutes. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF488A-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/500).
ab6313 staining Cathepsin D in HepaRG cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 in PBS and blocked with 1% milk at room temperature for 20 minutes. Samples were incubated with primary antibody (1µg/ml in 1% milk) for 30 minutes. An undiluted Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells
This product has been referenced in:
- Magalhaes J et al. Effects of ambroxol on the autophagy-lysosome pathway and mitochondria in primary cortical neurons. Sci Rep 8:1385 (2018). WB ; Mouse . Read more (PubMed: 29362387) »
- Duta-Mare M et al. Lysosomal acid lipase regulates fatty acid channeling in brown adipose tissue to maintain thermogenesis. Biochim Biophys Acta 1863:467-478 (2018). IHC-P ; Mouse . Read more (PubMed: 29374543) »