Product nameAnti-Cathepsin D antibody [EPR3054] - BSA and Azide free
See all Cathepsin D primary antibodies
DescriptionRabbit monoclonal [EPR3054] to Cathepsin D - BSA and Azide free
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cathepsin D aa 1-100. The exact sequence is proprietary.
- Human breast ductal infiltrating carcinoma tissue; A431, MCF7 and SKBR3 cell lysates.
ab206472 is the carrier-free version of ab134169 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab206472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 1.16 x 10 -10 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab206472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Tissue specificityExpressed in the aorta extrcellular space (at protein level).
Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
Sequence similaritiesBelongs to the peptidase A1 family.
Contains 1 peptidase A1 domain.
modificationsN- and O-glycosylated.
Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
- Information by UniProt
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
All lanes : Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free (ab206472) at 1/2000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : CTSD knockout A431 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 44 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
ab134169 was shown to recognize in wild-type A431 cells as signal was lost at the expected MW in CTSD knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTSD knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab134169 and ab7291 (Mouse anti-tubulin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134169).
ab206472 has not yet been referenced specifically in any publications.